Capital HPLC

Capital Optimal -brands

The Next Step Forward in Silica Engineering

 

The Next Step Forward in Silica Engineering The new range of Optimal® HPLC columns from Capital HPLC respresents the next step forward in porous silica engineering beyond the so-called new generation high purity materials.
The current new generation materials are based on an alternative synthesis route which yields a high purity product with excellent inertness characteristics. However, Optimal® is manufactured using an entirely new process based on well established, tried and tested silica production techniques coupled with new refining technologies which ultimately leads to a superior material combining the advantages of both the new and traditional approaches to silica manufacturing.

 
 Specifications

The fact that high purity Optimal is manufactured using well established silica engineering techniques means that a wide range of specifications are possible. As a result, we can supply Optimal in pore sizes ranging from 60Å to 1000Å with the most popular being 120Å. Moreover, Optimal is also available in particle sizes ranging from 3μm up to preparative grade sizes.

Shape:
Phases Available:
Particle Size:
Mean Pore Diameter:
Carbon Loading:
Specific Surface Area:
Spherical
ODS-H, ODS-L, C8 and Silica
3μm, 5μm and larger
120Å (300, 500 and 1000Å also available)
ODS-H (18%), ODS-L (15%)
320m2g-1. (120Å material)

Optimal® ODS-H

Optimal® ODS-H is the most generally applicable of the Optimal® phases and can be used for both specialist
applications involving acid and basic compounds or mixtures of both. Optimal® ODS-H is also particularly well
suited to general purpose method development.

         

   

The absence of active silanols in Optimal is illustrated by this
standard test mixture which includes both pyridine and phenol.
The lack of peak tailing clearly demonstrates the absence of
metallic impurities.

Column:                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:        40:60 Acetonitrile:Water
Temperature:        40°C
Flow Rate:             1ml/min
Detection:             UV at 254nm
                             1. Pyridine, 2. Phenol

 

   

Neutral silanol groups can still occasionally cause problems
with certain classes of compound. Theobromine, theophylline
and caffeine are particularly prone to this effect. In Optimal this
effect is elimated by full end capping.

Column:                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:        25:75 Methanol:Water
Temperature:        30°C
Flow Rate:             1ml/min
Detection:             UV at 254nm
                             

                             1. Theobromine, 2. Theophylline
                             3. Caffeine, 4. Phenol

 

Many compounds such as polyphenols interact irreversibly with the active sites on silica based reversed
phase materials. In the case of Optimal ODS-H this problem does not occur, which makes it possible
to analyse problematic compounds such as in the example below.

 

Column:
                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:        25:75 Methanol:Water
Temperature:        30°C
Flow Rate:             1ml/min
Detection:             UV at 254nm

                             

                             

 

 

Silianol Sensitive Vitamins

Column:                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:       15:85 Acetonitrile:Water
                             10mM Sodium Phosphate (pH5)

Temperature:        40°C
Flow Rate:             1ml/min
Detection:             UV at 254nm
                                                       

                             1. Thiamine Hydrochloride
                             2. Riboflavine

 

 

 

Tricyclic Antidepressives

Column:                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:       80:20 Methanol:Water
                             20mM Potassium Phosphate (pH 7)

Temperature:        40°C
Flow Rate:             1ml/min
Detection:             UV at 254nm
                                                                                  

                             1. Protriptyline
                             2. Nortriptyline
                             3. Amitriptyline

 

 

 

 

 

 

 Optimal® ODS-L

Optimal® ODS-L has a slightly lower carbon loading (15%) than the ODS-H and is useful for the analysis of a wide range of hydrophilic compounds which are normally not retained by other C18 based packing material.

One particular advantage is that even pure water can be used as the eluent without the need for any organic modifier. Highly polar samples only soluble in water may be separated by this packing material, e.g. sugars and other saccharides.

Optimal ODS-L is also as useful for analysing hydrophobic samples which are currently chromatographed on competitor C8 materials. Like ODS-H, this packing material is also fully endcapped. OptimalODS-L is also based on the same high purity silica as the Optimal ODS-H material which ensures the high degree of inertness and lack of peak tailing.

 

Fructo-Oligosaccharides

Column:                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:       100% Water (HPLC Grade)
Temperature:       40°C
Flow Rate:             1ml/min
Detection:             Refractive Index

                                                                                                      

                             1. Sucrose
                             2. Kestose
                             3. Fructotriose
                             4. Fructotetraose

 

 

 

 

 

 

 

Optimal Ordering Information

Catalogue numbers refer to 5μm materials 4.6mm column i.d. only. For 2.1mm or 3.2mm
diameter columns please add D2 and D3 respectively to the standard 4.6mm i.d. columns.
Prices for 2.1mm and 3.2mm i.d. columns are as for 4.6mm i.d.

New Precision Format Columns
5mm prices only (3μm and 10μm particles are also available)

 

        Phase               2cm          5cm          10cm          15cm         20cm         25cm
                              (guard)
        Silica            5SA102      5SA105      5SA110       5SA115      5SA120     5SA125
         C8                5SF102      5SF105      5SF110       5SF115      5SF120      5SF125
       ODS-H            5SG102     5SG105      5SG110       5SG115     5SG120       5SG125
       ODS-L            5SH102     5SH105      5SH110       5SH115      5SH120      5SH125
      PRICE/£          See price list page 1

 


UCCCartridgeFormatColumns
5µm prices only (3µmand 10µmparticles are also available)

 

        Phase               2cm          5cm          10cm          15cm         20cm         25cm
                              (guard)
        Silica             5SA102      5SA305      5SA310       5SA315     5SA320     5SA325
         C8                5SF102      5SF305      5SF310       5SF315       5SF320     5SF325
       ODS-H            5SG102     5SG305      5SG310       5SG315      5SG320     5SG325
       ODS-L            5SH102      5SH305      5SH310       5SH315      5SH320     5SH325
      PRICE/£          See price list page 1

 

 

 Wide Pore Materials


All Capital HPLC Optimal columns are available in various pore sizes ranging from 60Å up to
1000Å. This makes it possible to analyse larger molecules together with all the advantages of
a high purity, highly inert material. For prices and ordering information for wide pore materials,
please consult our price list, page 1.

 

 

Capital Inertpak-brands

Capital Inertpak

 

The Inertpak family of LC columns from Capital HPLC is based on a new generation high purity silica which
exhibits a remarkable degree of chemical inertness and stability. The Inertpak range is therefore particularly
effective in the analysis of amines, basic pharmaceuticals andotherchemicalspecieswherehydrogenbondingmay
occur. This also makes Inertpak columns generally suitable for the vast range of non-basic applications
currently performed on older technology stationary phases.

In the reversed phaseanalysis of basic /aminecompounds,severe peak tailing often occurs, and is mainly due to the material.
This leads to strong electrostatic interactions 
with the electron deficient regions of basic molecules resulting in peak tailing.


The tendency for a silanol group to undergo self ionisation depends on its electronic environment and is exceptionally strong when the adjacent silicon atom is substituted by a metallic impurity. Figures 1 and 2 above
illustrate the stabilising effect of metal impurities on ionised silanols.

Existing silica technology produces LC packing materials
which contain substantial amounts of metallic impurities,

 

Figure 1
Free Silanol (Moderately Acidic)

 

            Figure 2
            Metal Activated Silanol (Highly Acidic)

 

presence of surface silanol groups which remain after the
derivatisation procedure. End capping with trimethyl silyl
groups helps to eliminate surface silanols, but is never
completely resolves the problem.
The material used for the Capital HPLC Inertpak column
family represents an innovative solution to the problem
of residual silanol interactions.
Surface silanols are known to undergo self-ionisation
leading to fixed negative charges on the surface of the

notably sodium and iron which lead to the presence of
highly activated silanols which contribute to peak tailing.
The inert silica used in Inertpak columns uses a synthesis
route producing a high purity material with a very low
metal content. Consequently residual silanols following
the end capping process are inactive and therefore do not
create the interactions which cause peak tailing.

 

 

Specifications

Phases:

Particle Diameter:

Carbon Loading

 






: Spherical

: 5 micron

: C8(10.5%)
: C4(7.5%)
: Ph(10.0%)
: ODS2(18%)
: ODS-80Å(17.5%)

 

Mean Pore Diameter                 : ODS2 (150Å or 80Å)
                                                  : Ph,C8,C4 (150Å)
Surface Area                             : 320-350m2/g
                                                  : 450m2/g (ODS-80Å)
Assured Efficiency                    : 55,000 plates/m
                                                  (Typically 75,000)

 

 - Chemically Inert
- Excellent for Basic Compounds
- High Efficiency
- Good Peak Shape
- Stable over a Wide pH Range

 

 

 

In addition to the inertness towards basic compounds, Inertpak columns can also be operated over a wider pH range than conventional materials. The above tests illustrate the good stability of Inertpak in contact with aggressive mobile phases at high and low pH values.

New packing materials create morestring entrequirements when it comes to packing procedures. The proprietary packingprotocol, designed by our development laboratory, allows us to supply columns in all formats from capillaries to preparative scale columns, exclusive to Capital HPLC.
This packing procedure, in concert with Capital HPLC's stringent quality control assures you of reproducible cost effectiveanalysis.
Amitryptyline, the antidepressive drug, is known to be particularly sensitive to the presence of active silanol groups when analysed by reversed phase LC.
The Inertpak Inertness Test shows a comparison of the analyses of this compound on a conventional ODS; a leading brand base deactivated material; and Inertpak.

In the case of the conventional ODS material, the drug is irreversibly adsorbed onto the packing material and does not elute, whereas the base deactivated material elutes the drug with severe peak tailing. With Inertpak a perfectly formed symmetrical peak is observed.

 

 

 

 

 

 

 

 INERTNESS TEST

 

Inertpak Ordering Information
Catalogue numbers refer to 5μm materials 4.6mm column i.d. only. For 2.1mm or 3.2mm diameter
columns please add D2 and D3 respectively to the standard 4.6mm i.d. columns. Prices for 2.1mm and 
3.2mm i.d. columns are as for 4.6mm i.d.


Conventional Column Format
5μm prices only

        Phase              2cm             5cm          10cm          15cm            20cm          25cm
                              (guard)
         Silica            5MA102      5MA105      5MA110      5MA115       5MA120      5MA125
         C4                 5MD102      5MD105      5MD110      5MD115       5MD120     5MD125
         C8                 5MF102      5MF105      5MF110       5MF115       5MF120       5MF125
         ODS-2           5MG102      5MG105      5MG110      5MG115       5MG120      5MG125
         Phenyl(Ph)   5MK102      5MK105      5MK110       5MK115       5MK120      5MK125
         ODS(80Å)      5ML102      5ML105      5ML110       5ML115       5ML120      5ML125
       PRICE/£ see price list - page 3

 

 

Universal Cartridge Columns (UCC)
5μm prices only

 

        Phase              2cm             5cm          10cm          15cm            20cm          25cm
                              (guard)
         Silica             5MA102      5MA305      5MA310      5MA315       5MA320        5MA325
         C4                  5MD102      5MD305      5MD310      5MD315       5MD320       5MD325
         C8                  5MF102      5MF305      5MF310       5MF315       5MF320         5MF325
         ODS-2            5MG102      5MG305      5MG310      5MG315       5MG320        5MG325
         Phenyl(Ph)    5MK102      5MK305      5MK310       5MK315       5MK320        5MK325
         ODS(80Å)      5ML102      5ML305       5ML310       5ML315       5ML320        5ML325
       PRICE/£ see price list - page 3

 

 

 

 

Bases of Nucleic acid and Nucleosides

Column : Inertpak ODS2 5μm
150 x 4.6 mm I.D.
Eluent :
0.1M H3PO4 + 0.2M NaClO4 (pH2)


Flow rate : 1.0 ml/min.
Detector : UV 260nm


1. Cytosine         2. Uracil
3. Guanine          4. Adenine
5. Cytidine          6. Uridine
7. Thymine         8. Adenosine
9. Guanosine      10.Thymidine

 

 

 

 

 

 

 

Capital Spherisil-brands

Capital Spherisil

Spherisil HPLC columns and UCC Cartridges from Capital HPLC represent a 100% compatible alternative to Waters Spherisorb columns for all HPLC applications. Compare for yourself the chromatograms below which show the identical results obtained with both Capital Spherisil and Waters Spherisorb for compounds of vastly differing polarities. Existing Spherisorb methods
can be directly carried out on Capital Spherisil using exactly the same chromatographic run conditions with no changes in selectivity or retention characteristics.

Capital Spherisil is available in the following phases: ODS1, ODS2, C8, Cyano, Amino, Phenyl, SCX and SAX. Mixed mode phases are also available. All phases are available in 10, 5 and 3μm particle diameters

All Capital Spherisil columns must undergo the most stringent quality control conditions in our ISO9002 approved laboratory. Spherisil columns provide the same chromatographic performance as Spherisorb with lower column back pressures thanks to Capital's proprietary packing technique.
All materials are held in stock for immediate delivery from the narrow bore scale through to preparative columns.
In all test Spherisil was shown to be virtually indistinguishable from Waters Spherisorb except for
one important factor - price !!!
Compare the examples below with the examples
opposite.

Capital-Spherisil

 

 

 

 

 

 

 


Specifications

Phases:                              ODS1, ODS2, C8, C6, C1
                                          CN, NH2, Phenyl, Mixed
Mode: 
Particle Diameter:               3, 5 and 10μm
Pore Diameter:                    80 Â
Surface Area:                      220m2 / g



 

 

 Waters Spherisorb ODS1

Run Conditions: 15cm x 4.6mm i.d. 40:60 Acetonitirle:Water (20mM Phosphate pH 2.5)

 

Uracil, Phenol, Cresol, 3,5 Xylenol
and 2,5 Xylenol
Uracil, Aniline, Cresol,
Nitrobenzene and Toluene
Cinamic Acid, Nitrobenzene,
Amitriptyline and Biphenyl
      

 

   Phase                     2cm             5cm            10cm              15cm            20cm             25cm
                                (guard)
     Si                       5UA102        5UA105        5UA110        5UA115         5UA120         5UA125
     C1                      5UB102        5UB105        5UB110        5UB115          5UB120         5UB125
     C6                      5UE102        5UE105         5UE110        5UE115          5UE120         5UE125
     C8                      5UF102        5UF105         5UF110         5UF115          5UF120         5UF125
     ODS1                 5UG102        5UG105        5UG110         5UG115         5UG120         5UG125
     ODS2                 5UH102        5UH105        5UH110        5UH115         5UH120         5UH125
     PHENYL              5UK102        5UK105        5UK110         5UK115         5UK120         5UK125
     CN                      5UL102        5UL105        5UL110         5UL115          5UL120         5UL125
     NH2                   5UM102       5UM105        5UM110        5UM115        5UM120         5UM125

 

 The example below shows the analysis of the same samples under the same run conditions on Capital's Spherisil

 Capital Spherisil ODS1

Run Conditions: 15cm x 4.6mm i.d. 40:60 Acetonitirle:Water (20mM Phosphate pH 2.5)

Uracil, Phenol, Cresol, 3,5 Xylenol
and 2,5 Xylenol

Uracil, Aniline, Cresol,
Nitrobenzene and Toluene

Cinamic Acid, Nitrobenzene,
Amitriptyline and Biphenyl

      

UCC Cartridge Columns

5μm prices only
3μm columns 10% extra          - Replace first digit (i.e., 5) with 3 for cat. no.
10μm columns 5% less            - Replace first digit (i.e., 5) with 10 for cat. no.

   Phase                     2cm             5cm            10cm              15cm            20cm             25cm
                                (guard)
     Si                       5UA302        5UA305        5UA310        5UA315         5UA320         5UA325
     C1                      5UB302        5UB305        5UB310        5UB315          5UB320         5UB325
     C6                      5UE302        5UE305         5UE310        5UE315          5UE320         5UE325
     C8                      5UF302        5UF305         5UF310         5UF315          5UF320         5UF325
     ODS1                 5UG302        5UG305        5UG310         5UG315         5UG320         5UG325
     ODS2                 5UH302        5UH305        5UH310        5UH315         5UH320         5UH325
     PHENYL              5UK302        5UK305        5UK310         5UK315         5UK320         5UK325
     CN                      5UL302        5UL305        5UL310         5UL315          5UL320         5UL325
     NH2                   5UM302       5UM305        5UM310        5UM315        5UM320         5UM325

Spherisil ODS2

The adjacent test shows the comparison of Capital Spherisil ODS2 with Waters Spherisorb ODS2. As
with the ODS1 the results are identical in terms of selectivity and retention characteristics.

 Waters Spherisorb ODS2

Uracil, Aniline, Cresol,
Nitrobenzene, Toluene

Uracil, Phenol, Cresol,
3,5-Xylenol, 2,5-Xylenol

Uracil, 2-Nitroaniline
Methyl Benzoate, Xylene

      

Type-C Silica

Type-C Silica

 

 

 

 

 

 

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