Capital HPLC

Capital Spherisil-brands

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Published on 11 March 2013
Written by Super User
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Capital Spherisil

Spherisil HPLC columns and UCC Cartridges from Capital HPLC represent a 100% compatible alternative to Waters Spherisorb columns for all HPLC applications. Compare for yourself the chromatograms below which show the identical results obtained with both Capital Spherisil and Waters Spherisorb for compounds of vastly differing polarities. Existing Spherisorb methods
can be directly carried out on Capital Spherisil using exactly the same chromatographic run conditions with no changes in selectivity or retention characteristics.

Capital Spherisil is available in the following phases: ODS1, ODS2, C8, Cyano, Amino, Phenyl, SCX and SAX. Mixed mode phases are also available. All phases are available in 10, 5 and 3μm particle diameters

All Capital Spherisil columns must undergo the most stringent quality control conditions in our ISO9002 approved laboratory. Spherisil columns provide the same chromatographic performance as Spherisorb with lower column back pressures thanks to Capital's proprietary packing technique.
All materials are held in stock for immediate delivery from the narrow bore scale through to preparative columns.
In all test Spherisil was shown to be virtually indistinguishable from Waters Spherisorb except for
one important factor - price !!!
Compare the examples below with the examples
opposite.

Capital-Spherisil

 

 

 

 

 

 

 


Specifications

Phases:                              ODS1, ODS2, C8, C6, C1
                                          CN, NH2, Phenyl, Mixed
Mode: 
Particle Diameter:               3, 5 and 10μm
Pore Diameter:                    80 Â
Surface Area:                      220m2 / g



  

 

 Waters Spherisorb ODS1

Run Conditions: 15cm x 4.6mm i.d. 40:60 Acetonitirle:Water (20mM Phosphate pH 2.5)

 

Uracil, Phenol, Cresol, 3,5 Xylenol
and 2,5 Xylenol		 Uracil, Aniline, Cresol,
Nitrobenzene and Toluene		 Cinamic Acid, Nitrobenzene,
Amitriptyline and Biphenyl
      

 

   Phase                     2cm             5cm            10cm              15cm            20cm             25cm
                                (guard)
     Si                       5UA102        5UA105        5UA110        5UA115         5UA120         5UA125
     C1                      5UB102        5UB105        5UB110        5UB115          5UB120         5UB125
     C6                      5UE102        5UE105         5UE110        5UE115          5UE120         5UE125
     C8                      5UF102        5UF105         5UF110         5UF115          5UF120         5UF125
     ODS1                 5UG102        5UG105        5UG110         5UG115         5UG120         5UG125
     ODS2                 5UH102        5UH105        5UH110        5UH115         5UH120         5UH125
     PHENYL              5UK102        5UK105        5UK110         5UK115         5UK120         5UK125
     CN                      5UL102        5UL105        5UL110         5UL115          5UL120         5UL125
     NH2                   5UM102       5UM105        5UM110        5UM115        5UM120         5UM125

 

 The example below shows the analysis of the same samples under the same run conditions on Capital's Spherisil

 Capital Spherisil ODS1

Run Conditions: 15cm x 4.6mm i.d. 40:60 Acetonitirle:Water (20mM Phosphate pH 2.5)

Uracil, Phenol, Cresol, 3,5 Xylenol
and 2,5 Xylenol

Uracil, Aniline, Cresol,
Nitrobenzene and Toluene

Cinamic Acid, Nitrobenzene,
Amitriptyline and Biphenyl
      

UCC Cartridge Columns

5μm prices only
3μm columns 10% extra          - Replace first digit (i.e., 5) with 3 for cat. no.
10μm columns 5% less            - Replace first digit (i.e., 5) with 10 for cat. no.

   Phase                     2cm             5cm            10cm              15cm            20cm             25cm
                                (guard)
     Si                       5UA302        5UA305        5UA310        5UA315         5UA320         5UA325
     C1                      5UB302        5UB305        5UB310        5UB315          5UB320         5UB325
     C6                      5UE302        5UE305         5UE310        5UE315          5UE320         5UE325
     C8                      5UF302        5UF305         5UF310         5UF315          5UF320         5UF325
     ODS1                 5UG302        5UG305        5UG310         5UG315         5UG320         5UG325
     ODS2                 5UH302        5UH305        5UH310        5UH315         5UH320         5UH325
     PHENYL              5UK302        5UK305        5UK310         5UK315         5UK320         5UK325
     CN                      5UL302        5UL305        5UL310         5UL315          5UL320         5UL325
     NH2                   5UM302       5UM305        5UM310        5UM315        5UM320         5UM325

Spherisil ODS2

The adjacent test shows the comparison of Capital Spherisil ODS2 with Waters Spherisorb ODS2. As
with the ODS1 the results are identical in terms of selectivity and retention characteristics.

 Waters Spherisorb ODS2

Uracil, Aniline, Cresol,
Nitrobenzene, Toluene

Uracil, Phenol, Cresol,
3,5-Xylenol, 2,5-Xylenol

Uracil, 2-Nitroaniline
Methyl Benzoate, Xylene
      
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