Capital HPLC

MonoFAS DNA Purification Kit I

MonoFas DNA Purification Kit I

 

MonoFas DNA Purification Kit I purifies
DNAfrom the PCR products and agarose gels.
Not only purifies the fragment DNA directly from
the PCR liquid, but MonoFas DNA Purification
Kit I also purifies the DNA from standard or low
melting agarose gels (TAE or TBE) slices.
MonoFas DNA Purification Kit I can be applied
for both centrifugation and aspiration methods.
 
   

Features
Fast


DNA purification from PCR samples in 4
minutes.


DNA purification from agarose gels in 9 minutes.
Small Sample Volume No damages on DNA by a strong centrifuge with monolith technology. Samples can be as small as10μL and more than 90% recovery rate.

High Nucleic Acid Retention The high retentivity of the Monolithic silica against nucleic acid enables high purification and recovery. The wide range of fragment DNA between 35bp - 35kp can be purified.
Product Contents (MonoFas DNA Purification Kit I 50 cs.)

Spin Column              50 pcs
Colelction Tube         50 pcs
Bind BufferA              30 mL
Wash BufferB            40 mL
Elute BufferC             10 mL
Operation Manual      1 pcs

 

 

 

 

 

 



Items


    Purificatiofrom PCR


urificatiofrom agarose g


                       Time

   4minutes

9minutes

·     Binding Amoun

                  10μg

10μ

·     Maximum Gel Throughput

1g

·     inimuElutionAmoun

10μL

10μL

·     ColumVolume

1mL

1mL

·     ProcessablDNRang

           
          35bp35kbp


              35bp35kbp

·            RecoveryRate

>90% (100bp5kbp)

>80(100bp5kbp)

>60% (5kbp35kbp)

>50(5kbp35kbp)

·     imeEliminatioRat

95%

                                                                   
                                                                             

 

High throughput rate

A maximum throughput of 1g of agarose gel
slices means that a large number of gel slices
can be rapidly extracted.

M: pHY Marker
1: Unpurified PCR sample
2: Purified from Agarose Gel (MonoFas I)

 

 

 

Highly Effective Ligation
Lane 1:
Target gene (ARL1) purified by MonoFas I was
integrated into the bector, then amplified and
extracted.
Lane 2:
Confirming the target gene by restriction and
endonuclease treatment.
MonoFasI can increase the ligation efficiency as
it highly purifies the fragment DNA. Also neither
ethanol precipitation nor phenol extraction
before ligation is necessary.

M: phY Marker
1: Plasmid DNA
2: Plasmid DNA after restricting enzyme.

 

MonoFas I (Purified fragment DNA from Agarose Gel)

 

Condition:       Cycle sequencing by the Big Dye Terminator v3.1 manufactured by ABI
Analyzer:        ABI 3 7 3 0 Genetic Analyzer

Wide Adaptive Range of Fragment DNA Sizes


Due to the strong retention ability for nucleic acid, MonoFas I can effectively purify the fragment
DNA in a wide range from 35bp to 35kbp)

 

 

Recovery rate by Elution Volume and DNA Size

Recovery rate was examined with 400bp fragment DNA

Elution

Vo lume

Recovery

Rate

5uL

83%

10uL

89%

15uL

91%

25uL

88%

50uL

83%

2000bp

94%

5000bp

92%

10000bp

96%

15000bp

96%

20000bp

95%

30000bp

92%

DNASi ze

Recovery

Rate

35bp

65%

80bp

80%

100bp

85%

500bp

93%

1000bp

96%

2000bp

94%

5000bp

92%

10000bp

96%

15000bp

96%

20000bp

95%

30000bp

92%

How to use the Adaptor

 

Please use the microtube centrifige adaptor when using a 2.2ml centrifuge machine

Item

Volume

Cat.No.

  • MonoFas mini DNA Blood KitⅡ
    • batch Buffer included
 

5010-21512

  • Micro Tube Centrifuge Adaptor

6pcs

5010-21513

 

 

 

 

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