MonoFAS Plasmid Extraction Kit III
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- Category: p11
- Published on 29 May 2013
- Written by Super User
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MonoFas Plasmid Extraction Kit III
MonoFas Plasmid Extraction Kit III quickly and |
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Product Contents
(MonoFas Plasmid Extraction Kit III 100pcs)
Item |
Volume |
|
100 pcs |
|
100 pcs |
|
25mL |
|
25mL |
|
35mL |
|
60mL |
|
12mL |
|
400μL |
|
1 pcs |
Specification
Item |
Volume |
ThroughputVolumeofCultureSolution |
1~3mL |
HighCopiedPlasmidYield |
15μg/mL culture |
LowCopiedPlasmidYield |
5μg/mL culture |
RecommendedElutionVolume |
50~100μL |
DNA Purity(O.D.260/280) |
1.7~1.9 |
ColumnVolume |
1mL |
Plasmid Extraction Comparison
MonoFas Plasmid Extraction Kit III produces a
much purer extraction than other similar
products. This is due to the structure of the
silica monolith, which does not need extra
pressure and the large surface areas for better
nucleic acid absoption.
Stable Recovery Rate
Due to the strict lot control of the silica monolith
that retains nucleic acid, the same amount of
Plasmid can be extracted from the same
bacteria coli culture solution.
The picture above shows the extraction of
2.6kb low copy Plasmid in JM 109 bacteria
coli and the recovery rate.
Extractable from BAC Clone
Usable for Restriction Enzyme
Digestion
Extract the Plasmid DNA containing the ARL 1
gene from bacteria coli (DH5a) using MonoFas
(Lanes 1 - 3). Digest the extracted plasmid with
the restriction enzyme EcoRI-HindIII and confirm
the target gene (ARL 1:581bp) (lanes 4 - 6).
Usable for Sequencing
Sequence analyses using the extracted plasmid.
Sample: DNA to make it amplify from human genome
by using Takara PCR Kit.
Sequence: data analyzed with ABI Prism 3730 x I
Genetic Analyzer
Item |
Batch |
Cat.No. |
Price |
|
5010-21533 |
||
|
100 |
5010-21534 |
|
|
250 |
5010-21535 |