Capital HPLC

MonoFAS Plasmid Extraction Kit III

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Category: p11
Published on 29 May 2013
Written by Super User
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MonoFas Plasmid Extraction Kit III

 

MonoFas Plasmid Extraction Kit III quickly and
easily extracts highly pure plasmid DNA from the
E.Coli cultivated solution by using the centrifuge
method and the adsorption of DNA on Silica
Monolith. The extracted plasmid DNA can be
directly used for the restriction enzyme
treatment, ligation, sequence, PCR amplification
and transfection.

  
   

Fast

 
Only 8 minutes to extract the plasmid DNA per
sample with the MonoFas Plasmid Extraction
Kit III
Excellent Retention of Nucleic
By using the Silica Monolith technology,
MonoFas Plasmid Extraction Kit III extracts
highly pure plasmid DNA. Therefore there is no
need for ethanol precipitation and chloroform
extraction after the DNA extraction.
Purification of BAC Clone
With the large surface of the Silica Monolith,
DNA can be also purified from BAC Clone.

 

 

 

Product Contents
(MonoFas Plasmid Extraction Kit III 100pcs)

 

Item

Volume
  •  Spin Column

       100 pcs

 

  • Collection Tube
       100 pcs
 
  • Buffer A-3 (Vortex）

 

        25mL
  • Buffer B-3 (Bacteriolysis）

 

        25mL
  • Buffer C-3 (Binding・Absorpt

 

        35mL
  • Buffer D-3 (Washing）

 

       60mL
  • Buffer E-3 (Elution）

 

       12mL
  • RNase A

 

       400μL
  • Operation Manual
    • pcs
        
       1 pcs

 

 

Specification

Item

Volume

ThroughputVolumeofCultureSolution

1～3mL

HighCopiedPlasmidYield

15μg/mL culture

LowCopiedPlasmidYield

5μg/mL culture

RecommendedElutionVolume

50～100μL

DNA Purity(O.D.260/280)

1.7～1.9

ColumnVolume

1mL

 

 Plasmid Extraction Comparison

MonoFas Plasmid Extraction Kit III produces a
much purer extraction than other similar
products. This is due to the structure of the
silica monolith, which does not need extra
pressure and the large surface areas for better
nucleic acid absoption.

Stable Recovery Rate

 

Due to the strict lot control of the silica monolith
that retains nucleic acid, the same amount of
Plasmid can be extracted from the same
bacteria coli culture solution.
The picture above shows the extraction of
2.6kb low copy Plasmid in JM 109 bacteria
coli and the recovery rate.

Extractable from BAC Clone

Usable for Restriction Enzyme
Digestion
Extract the Plasmid DNA containing the ARL 1
gene from bacteria coli (DH5a) using MonoFas
(Lanes 1 - 3). Digest the extracted plasmid with
the restriction enzyme EcoRI-HindIII and confirm
the target gene (ARL 1:581bp) (lanes 4 - 6).

Usable for Sequencing

Sequence analyses using the extracted plasmid.

 

Sample: DNA to make it amplify from human genome
by using Takara PCR Kit.
Sequence: data analyzed with ABI Prism 3730 x I
Genetic Analyzer

Item

Batch

Cat.No.

Price
  1. MonoFasPlasmid Extraction KitⅢ
    1. 50
  

5010-21533

  
  1. MonoFasPlasmid Extraction KitⅢ

100

5010-21534

  
  1. MonoFasPlasmid Extraction KitⅢ

250

5010-21535

  

 

 

 

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