Capital HPLC

Anodic Flow Capillaries

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Category: p9
Published on 25 May 2013
Written by Super User
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Anodic Flow Capillaries

From the earliest days of CE, it was realised that the poor peak shapes which were frequently observed with bases and biomolecules were mainly the result of interaction between the analyte and the capillary walls. Often protein separations were carried out at

high pH well above their isoelectric points in order that the wall and the analyte were both negatively charged.

Although effective for some separations, this approach effectively removes choice of pH as a separation variable.

In order to overcome this, EKT anodic flow capillaries have a permanent positive charge built into the polymer coating. This permits the separation of bases and basic proteins over a wider pH range (see application) below.

Due to the presence of the fixed positive charges the EOF is in the opposite direction to that of bare silica. (see opposite).

The separation of the basic proteins shown in the example below would normally have to be carried out at alkaline pH values in order to try and ensure that the proteins and the capillary wall would be negatively charged.

 

 

The example below illustrates the possibilities of using EKT
anodic flow capillaries for the analysis of proteins at acid
pH values. Not only does the use of anodic flow capillaries,
as opposed to bare silica capillaries, result in a vastly
improved peak shape, but the elution order of the
components lysozyme, cytochrome C, myoglobin and
ribonuclease A is completely reversed.

 

 Improved Peaks shapes for Basic Proteins



Anodic Flow Coated Capillaries

The open format capillaries are supplied precut to the required size with the UV window
correctly positioned to fit the specific instrument manufacturer's cartridge system. The capillary
ends are expertly cut to ensure precise sampling.

Cat.No.

Description

Price/item

Packof3

EKK1125

25cmCoatedCapillary(AnodicFlow)75µm

    

EKK1150

EKK1175

50cmCoatedCapillary(AnodicFlow)75µm

75cmCoatedCapillary(AnodicFlow)75µm

    

EKL1125

25cmCoatedCapillary(AnodicFlow)50µm

    

EKL1150

EKL1175

50cmCoatedCapillary(AnodicFlow)50µm

75cmCoatedCapillary(AnodicFlow)50µm

    



Anodic Flow Coated Capillaries - Cartridge Refit Service
Beckman Format Total length is nominal length plus 7cm after detection window

Cat.No.

Description

Price/item

Packof3

EKK1225

Beckman20cmAnodicFlow75µ m

    

EKK1250

EKK1275

Beckman50cmAnodicFlow75µ m

Beckman70cmAnodicFlow75µ m

    

EKL1125

Beckman20cmAnodicFlow50µ m

    

EKL1150

EKL1175

Beckman50cmAnodicFlow50µ m

Beckman70cmAnodicFlow50µ m

    



HP Format Capillary fitted to Hewlett-Packard cartridge

Cat.No.

Description

Price/item

Packof3

EKK1325

HP-20cmAnodicFlow 75µm

    

EKK1350

EKK1375

HP-50cmAnodicFlow 75µm

HP-70cmAnodicFlow 75µm

    

EKL1125

HP-20cmAnodicFlow 50µm

    

EKL1150

EKL1175

HP-50cmAnodicFlow 50µm

HP-70cmAnodicFlow 50µm

    

TSP Format capillary fitted to TSP cartridge

Cat.No.

Description

Price/item

Packof3

EKK1425

TSP - 20cm Anodic Flow 75μm

    

EKK1450

EKK1475

TSP - 50cm Anodic Flow 75μm

TSP - 70cm Anodic Flow 75μm

    

EKL1125

TSP - 20cm Anodic Flow 75μm

    

EKL1150

EKL1175

TSP - 50cm Anodic Flow 75μm

TSP - 70cm Anodic Flow 75μm

    

 



 

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