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Category: p9
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Published on 26 May 2013
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Written by Super User
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Buffer Preparation Reagents
0.1M Sodium Hydroxide
This reagent is widely used to rinse uncoated capillaries between injections in order to remove any adsorbed contaminants, which may otherwise cause variations in EOF, from the fused silica surface. The reagent is also used in order to reactivate uncoated fused silica capillaries before their initial use.
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250ml EKT511 100ml EKT510
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200mM Sodium Dodecyl Sulphate (SDS)
This solution is generally added to high pH (pH 7-10) buffers to form the micelles necessary for Micellar Electrokinetic Capillary Chromatography (MECC). The CE based separation of electrically neutral compounds is generally achieved using this technique.
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250ml EKT513 100ml EKT512
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100mM NaH2PO4 Phosphate Buffer adjusted to pH 2.5
This is a standard buffer for the separation of basic compounds. It has, for example, been used for the simultaneous resolution of 17 basic drugs. For the resolution of basic chiral compounds, such as pharmaceuticals and agrochemicals, cyclodextrins can be added to this buffer.
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250ml EKT515 100ml EKT514
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100mM Triethanolamine-Phosphoric Acid adjusted to pH 2.5
This is a useful alternative to pH 2.5 phosphate buffer for the separation of basic compounds. Compared with the standard phosphate buffer it can often give improved peak shape and resolution. Highly efficiency chiral separations of basic compounds can be achieved by the addition of cyclodextrins to this buffer.
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250ml EKT517 100ml EKT516
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100mM Phosphate Buffer adjusted to pH 7
( 50mm NaH2PO4 / 50mm Na2HPO4 )
This medium pH buffer is useful for the separation of a wide range of acidic compounds including agrochemicals, drugs and vitamins. It can be used in conjunction with the 100mM SDS solution (EKT513) to produce a medium pH micellar buffer.
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250ml EKT519 100ml EKT518
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100mM Borax (100mM Sodium Tetraborate)
This solution has a natural pH of 9.4 and is useful for the separation of a wide range of acidic compounds. It generates a higher EOF than the pH 7 phosphate buffer (cat. no.). Mixed with the SDS solution (cat no.) it gives rise to a high pH micellar electrolyte. The pH can be adjusted to higher values by the addition of 100mM sodium hydroxide solution (cat. no.). An additional feature of the borate buffer is the ability of the borate ions to chelate with various diol compounds leading to a modified or enhanced selectivity.
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250ml EKT521 100ml EKT520
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