Capital HPLC

Buffer Preparation Reagents

Buffer Preparation Reagents

0.1M Sodium Hydroxide                                                   

This reagent is widely used to rinse uncoated capillaries
between injections in order to remove any adsorbed 
contaminants, which may otherwise cause variations in
EOF, from the fused silica surface. The reagent is also
used in order to reactivate uncoated fused silica capillaries
before their initial use.

 

250ml     EKT511
100ml     EKT510

200mM Sodium Dodecyl Sulphate (SDS)                       

This solution is generally added to high pH (pH 7-10) buffers
to form the micelles necessary for Micellar Electrokinetic
Capillary Chromatography (MECC). The CE based
separation of electrically neutral compounds is generally
achieved using this technique.

 

250ml     EKT513
100ml     EKT512

100mM NaH2PO4 Phosphate Buffer adjusted to pH 2.5      

This is a standard buffer for the separation of basic
compounds. It has, for example, been used for the  
simultaneous resolution of 17 basic drugs. For the 
resolution of basic chiral compounds, such as
pharmaceuticals and agrochemicals, cyclodextrins can be
added to this buffer.

 

250ml       EKT515
100ml       EKT514

100mM Triethanolamine-Phosphoric Acid adjusted to pH 2.5  

This is a useful alternative to pH 2.5 phosphate buffer for  
the separation of basic compounds. Compared with the
standard phosphate buffer it can often give improved peak
shape and resolution. Highly efficiency chiral separations
of basic compounds can be achieved by the addition of
cyclodextrins to this buffer.

 

250ml         EKT517
100ml         EKT516

100mM Phosphate Buffer adjusted to pH 7                             

( 50mm NaH2PO4 / 50mm Na2HPO4 )

This medium pH buffer is useful for the separation of a
wide range of acidic compounds including agrochemicals,
drugs and vitamins. It can be used in conjunction with the
100mM SDS solution (EKT513) to produce a medium pH
micellar buffer.

 

250ml         EKT519
100ml         EKT518

100mM Borax (100mM Sodium Tetraborate)                           

This solution has a natural pH of 9.4 and is useful for the
separation of a wide range of acidic compounds. It
generates a higher EOF than the pH 7 phosphate buffer
(cat. no.). Mixed with the SDS solution (cat no.) it gives
rise to a high pH micellar electrolyte. The pH can be
adjusted to higher values by the addition of 100mM sodium
hydroxide solution (cat. no.). An additional feature of the
borate buffer is the ability of the borate ions to chelate with
various diol compounds leading to a modified or enhanced
selectivity.

 

250ml         EKT521
100ml         EKT520

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