Capital HPLC

NaAMPS Coated Capillaries

NaAMPS Coated Capillaries

In collaboration with Chemical Solutions in the USA, Electro-Kinetic Technologies have
introduced a unique anionic capillary coating based on a stable polymer incorporating
sulphonic acid functional groups (abbreviated as NaAMPS). This has made possible the
development of a range of capillaries with different, but pH independent EOF rates. Zero
flow capillaries are also available.

NaAMPS capillaries are manufactured under the strictest GMP/GLP conditions in order to
ensure reliability and excellent batch to batch reproducibility.
In contrast to most other coating materials, NaAMPS coated capillaries are extremely
stable and have a considerably long lifetime. 

This polymeric coating with surface sulphonic acid groups (NaAMPS coating) has, unlike
bare silica, a clearly defined pKa value. The surface is therefore fully ionised over a wide
pH range. The leads to a reasonably constant EOF over the same pH range.
The figure below shows the comparative EOF rates on bare silica and an EKT capillary with
NaAMPS coating.

Benefits of NaAMPS Coated Capillaries :

 

O- Decreased Sample - Wall Interactions

O-pH Independent Electro-Osmotic Flow

O-
Adjustable Electro-Osmotic Flow


O-
Reproducable Manufacturing Process


O-
Good Stability and Lifetime

 

 

Effect of pH on EOF for Bare Silica Compared to NaAMPS Coated Silica

When developing methods for bare silica CE by varying the buffer pH, it can become
confusing as changes in pH alter both the analtye electrophoretic mobility and the EOF.
The use of NaAMPS coated capillaries allows the user to easily assess the effect the change
of pH has on the selectivity for their samples without the inconvenience of EOF changes.

NaAMPS capillaries are available from EKT with 3 different surface charge densities which
enable the user to choose which degree of electro-osmotic flow is required.
NaAMPS Capillary Types

High Flow

Where a high electro-osmotic flow is required
over a large pH range.

Medium Flow
 Medium density surface charge, produces a moderate electro-osmotic flow

Low Flow
 Generates a low EOF and is particularly useful for isoelectric focussing avoiding the need
for complex and costly additives

 

In addition to the above NaAMPS coated capillaries. Electro-Kinetic Techologies also offer a
polyacrylamide coated zero flow capillary for use in applications where it is desirable to have zero
EOF and/or a neutral surface (see page 93).



NaAMPS Coated Capillaries (continued)

The use of NaAMPS coated capillaries has the following implications for CE and MECC method
development.

O-  Improves separation efficiency for biomolecules
O-  Use pH to adjust selectivity without affecting EOF
O-  Ideal for chiral separations
O-  Operate MECC over a wide pH range

The Use of NaAMPS Coated Capillaries in Micellar Electrokinetic Chromatography



Ordering Information

Low Flow NaAMPS Coated Capillaries

The open format capillaries are supplied precut to the required size with the UV window
correctly positioned to fit the specific instrument manufacturer's cartridge system. The capillary
ends are expertly cut to ensure precise sampling.

Open Format Please specify both effective length and total length when ordering.

Cat.No.

Description

Price/item

Packof3

EKE1125

0-35cm Coated Capillary (Low Flow) 75μm

   

EKE1150

35-65cm Coated Capillary (Low Flow) 75μm

   

EKE1175

70-100cm Coated Capillary (Low Flow) 75μm

   

EKF 1125

0-35cm Coated Capillary (Low Flow) 50μm

   

EKF1150
EKF1175

35-65cm Coated Capillary (Low Flow) 50μm
75-100cm Coated Capillary (Low Flow) 50μm

   

Low Flow NaAMPS Coated Capillaries - Cartridge Refit Service

Beckman Format Total length is nominal length plus 7cm after detection window.

Cat.No.

Description

Price/item

Packof3

EKE1225

Beckman-20cmCoatedCapillary(LowFlow)75µm

   

EKE1250

EKE1275

Beckman-50cmCoatedCapillary(LowFlow)75µm

Beckman-70cmCoatedCapillary(LowFlow)75µm

   

EKF1225

Beckman-20cmCoatedCapillary(LowFlow)50µm

   

EKF1250

EKF1275

Beckman-50cmCoatedCapillary(LowFlow)50µm

Beckman-70cmCoatedCapillary(LowFlow)50µm

   


H
PFormacapillary fitted to Hewlett-Packard Cartridge

Cat.No.

Description

Price/item

Packof3

EKE1325

HP-25cmCoatedCapillary(LowFlow)75µm

   

EKE1350

EKE1375

HP-50cmCoatedCapillary(LowFlow)75µm

HP-75cmCoatedCapillary(LowFlow)75µm

   

EKF1325

HP-25cmCoatedCapillary(LowFlow)50µm

   

EKF1350

EKF1375

HP-50cmCoatedCapillary(LowFlow)50µm

HP-75cmCoatedCapillary(LowFlow)50µm

   

 

        TSP Format Capillary Fitted to TSP Cartridge

Cat.No.

Description

Price/item

Packof3

EKE1425

TSP-25cmCoatedCapillary(LowFlow)75µm

   

EKE1450

EKE1475

TSP-50cmCoatedCapillary(LowFlow)75µm

TSP-75cmCoatedCapillary(LowFlow)75µm

   

EKF1425

TSP-25cmCoatedCapillary(LowFlow)50µm

   

EKF1450

EKF1475

TSP-50cmCoatedCapillary(LowFlow)50µm

TSP-75cmCoatedCapillary(LowFlow)50µm

   

 

 

High Flow NaAMPS Coated Capillaries

The open format capillaries are supplied precut to the required size with the UV window
correctly positioned to fit the specific instrument manufacturer's cartridge system. The capillary
ends are expertly cut to ensure precise sampling.

 

High Flow NaAMPS Open Format Please specify both effective length and total length
when ordering.

Cat.No.

Description

Price/item

Packof3

EKI1125

0-35cmCoatedCapillary(HighFlow)75µm

   

EKI1150

EKI1175

35-65cmCoatedCapillary(HighFlow)75µm

70-100cmCoatedCapillary(HighFlow)75µm

   

EKJ1125

0-35cmCoatedCapillary(HighFlow)50µm

   

EKJ1150

EKJ1175

35-65cmCoatedCapillary(HighFlow)50µm

75-100cmCoatedCapillary(HighFlow)50µm

   

Beckman Format Total length is nominal length plus 7cm after detection window.

High Flow NaAMPS Coated Capillaries - Cartridge Refit Service

BeckmanFormatTotallengthisnominallengthplus7cmafterdetectionwindow.


Cat.No.

Description

Price/item

Packof3

EKI1225

Beckman-20cmCoatedCapillary(HighFlow)75µm

   

EKI1250

EKI1275

Beckman-50cmCoatedCapillary(HighFlow)75µm

Beckman-70cmCoatedCapillary(HighFlow)75µm

   

EKJ1225

Beckman-20cmCoatedCapillary(HighFlow)50µm

   

EKJ1250

EKJ1275

Beckman-50cmCoatedCapillary(HighFlow)50µm

Beckman-70cmCoatedCapillary(HighFlow)50µm

   

 

HP Format Capillary fitted to Hewlett-Packard Cartridge

 

Cat.No.

Description

Price/item

Packof3

EKI1325

HP - 25cm Coated Capillary (High Flow) 75μm

   

EKI1350

EKI1375

HP - 50cm Coated Capillary (High Flow) 75μm

HP - 75cm Coated Capillary (High Flow) 75μm

   

EKJ1325

HP - 25cm Coated Capillary (High Flow) 50μm

   

EKJ1350

EKJ1375

HP - 50cm Coated Capillary (High Flow) 50μm

HP - 75cm Coated Capillary (High Flow) 50μm

   


TSPFormat
CapillaryfittedtoTSPcartridge.

Cat.No.

Description

Price/item

Packof3

EKI1425

TSP-25cmCoatedCapillary(HighFlow)75µm

   

EKI1450

EKI1475

TSP-50cmCoatedCapillary(HighFlow)75µm

TSP-75cmCoatedCapillary(HighFlow)75µm

   

EKJ1425

TSP-25cmCoatedCapillary(HighFlow)50µm

   

EKJ1450

EKJ1475

TSP-50cmCoatedCapillary(HighFlow)50µm

TSP-75cmCoatedCapillary(HighFlow)50µm

   

 

Medium Flow NaAMPS Coated Capillaries

The open format capillaries are supplied precut to the required size with the UV window
correctly positioned to fit the specific instrument manufacturer's cartridge system. The capillary
ends are expertly cut to ensure precise sampling.

Medium Flow NaAMPS Open Format Please specify both effective length and total length
when ordering.

Cat.No.

Description

Price/item

Packof3

EKG1125

0-35cmCoatedCapillary(MediumFlow)75µm

   

EKG1150

EKG1175

35-65cmCoatedCapillary(MediumFlow) 75µm

70-100cmCoatedCapillary(MediumFlow) 75µm

   

EKH1125

0-35cmCoatedCapillary(MediumFlow)50µm

   

EKH1150

EKH1175

35-65cmCoatedCapillary(MediumFlow) 50µm

75-100cmCoatedCapillary(MediumFlow) 50µm

   

 

Medium Flow NaAMPS Coated Capillaries - Cartridge Refit Service

Beckman Format Total length is nominal length plus 7cm after detection window

Cat.No.

Description

Price/item

Packof3

EKG1225

Beckman-20cmCoatedCapillary(Medium) 75µm

   

EKG1250

EKG1275

Beckman-50cmCoatedCapillary(Medium) 75µm

Beckman-70cmCoatedCapillary(Medium) 75µm

   

EKH1225

Beckman-20cmCoatedCapillary(Medium) 50µm

   

EKH1250

EKH1275

Beckman-50cmCoatedCapillary(Medium) 50µm

Beckman-70cmCoatedCapillary(Medium) 50µm

   

 

HPFormaCapillary fitted to Hewlett-Packard Cartridge

Cat.No.

Description

Price/item

Packof3

EKG1325

HP - 25cm Coated Capillary (Medium Flow) 75μm

   

EKG1350

EKG1375

HP - 50cm Coated Capillary (Medium Flow) 75μm

HP - 75cm Coated Capillary (Medium Flow) 75μm

   

EKH1325

HP - 25cm Coated Capillary (Medium Flow) 50μm

   

EKH1350

EKH1375

HP - 50cm Coated Capillary (Medium Flow) 50μm

HP - 75cm Coated Capillary (Medium Flow) 50μm

   

 

 

TSPFormat Capillary fitted to TSPcartridge

Cat.No.

Description

Price/item

Packof3

EKG1425

TSP-25cmCoatedCapillary(MediumFlow)75µm

   

EKG1450

EKG1475

TSP-50cmCoatedCapillary(MediumFlow)75µm

TSP-75cmCoatedCapillary(MediumFlow)75µm

   

EKH1425

TSP-25cmCoatedCapillary(MediumFlow)50µm

   

EKH1450

EKH1475

TSP-50cmCoatedCapillary(MediumFlow)50µm

TSP-75cmCoatedCapillary(MediumFlow)50µm

   

 

 

 

 

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