Capital HPLC

Diacel Chiral Phases

Diacel Chiral Phases

Diacel phases are popular for the enantiomeric separation of various types of chemical species.
They include phases from the Type II, Type III and Type IV chiral phase classifications.

Type II Diacel Phases 

These phases are based on modified cellulose (a natural polymer) generally coated onto silica.
The separations occur due to a multi-mode mechanism involving hydrogen bonding, p−p interactions,dipole stacking
and inclusion complexes. The phases contain chiral cavities or ravines which have a high affinity for aromatic groups.

Forseparations to occur there must be a tight fit of part of the solute into the cavity, and at least one of the
substituents on the chiral centre, needs to interact with the steric environment just outside the cavity.

Each derivative shows subtly different characteristics, and the series is capable of separating a very wide range of
compound classes including aliphatic compounds. Applications are generally for compounds possessing an aromatic,
carbonyl, nitro, sulfinyl, cyano or hydroxyl groups. Compounds containing either a carboxylic acid, or amine function
at the chiral centre, can be resolved after derivatization to a neutral compound, typically a benzylamide.

Whilst these stationary phases often have strict limitations on the composition of the mobile phase
(check manufacturers literature) it is the main influencing factor on chiral recognition. The typical
mobile phase is hexane/2-propanol, although often replacing the latter with ethanol (where permissible)
, will improve resolution. These phases have high capacity, and good efficiency, and are suitable for
preparative separations. The use of a guard column is recommended when working with biological samples.

Ordering Information

Please use the following catalogue numbers when ordering Type II chiral stationary phases. For
prices see our general price list page 17-18.

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