Capital HPLC

Capital Column Formats

 Conventional HPLC Columns

Unless specified otherwise, Capital HPLC supply high quality HPLC columns fitted with Parker female geometry fittings. Other types of end fitting are available or request. The standard internal diameter of the column is 4.6mm although other sizes can be supplied on request. The tubing itself consists of 316 stainless steel and is internally polished to a high degree. For chromatographers seeking stainless steel-free columns i.e.. for protein analysis, we can provide titanium, or PEEK, HPLC columns. Please contact our sales office for further information.

 

 

 

 

 

Capital HPLC Universal Cartridge Columns (UCC)

Universal Cartridge Columns (UCC)

Introduced in 1987, the UCC system is a high quality cartridge system which has been painstakingly optimized for flexibility and ease of handling. Unlike many of the over complicated cartridge systems available on the HPLC market our design has been kept simple so that we can offer a high quality product at a lower cost than a conventional column. The cartridge insert is simply a 1/4" stainless steel tube with KEL-F plugs at either end. The complete system is also entirely finger tight and does not require the use of wrenches or spanners for routine maintenance or changing cartridges etc.. Unlike most systems, the cartridge holders and inserts are compatible with standard 'off-the-shelf' HPLC end fittings and therefore do not require complex and expensive end assemblies. There is also arrange of easy to use accessories to enhance the basic UCC system. These include a simple guard column adapter and a convenient column coupler. The basic cartridge holder is not only compatible with 4.6mm i.d. columns, but also with narrow bore columns of 3.2 and 2.1mm internal diameter. Larger sized holders are also available for preparative and semi preparative cartridges. are also available for preparative and semi preparative cartridges.

New Precision Format

Capital HPLC's new specialty materials, Optimal and Inertpak are supplied in our stylish New Precision column ormat which combines high performance with ease of use. New Precision columns are machined to a very high degree of olerance and are available in all analytical dimensions. New Precision 1mm i.d. narrow bore columns deliver the ame performance in terms of plate number and peak symmetry as our standard 4.6mm columns.

However the chromatographer can enjoy all the benefits of narrow bore (1mm i.d.) columns in a robust and easy to use column format. The majority of 1mm bore column manufacturers supply 1mm columns in an 1/8" o.d. column format which leads to a rather fragile product which is easily bent and destroyed. However, New Precision 1mm i.d. columns have an external diameter of 1/4" (6.35mm) which means that they are robust enough to be handled just like conventional sized analytical columns.

 

Guard Columns ew Precision Cumns

 Capital HPLC guard columns (2cm in length) can be used with any of the above column formats. NB. For conventional and New Precision type columns a guard column holder (together with end fittings) is required

(Cat. No. HT7151 page 69). For our UCC cartridge column system, it is necessary to use a guard column adapter (Cat. No. HT7152 page 69).

Guard columns are available individually or alternatively in economy packs of three.

In addition to our standard 2cm guard columns, a 1cm PEEK guard column and holder is also available packed with the material required.

 

 

 10mm Guard Cartridge System

 Universal Integral Cap-Guard Cartridge System

 Capital HPLC has special on-column guard column system called universal on-column Cap guard system. These are used to extend the lifetime of your HPLC column by protecting against particulate matter and the elution of contaminants into the analytical column.

 The 10mm Guard Cartridges are easily and quickly inserted into the cartridge holder using Capital HPLC's finger-tight connectors. The compact size of the Universal Integral Cap-Guard Cartridge system makes it a convenient way to protect your analytical HPLC columns.

Guard cartridge availability

 

To connect the guard cartridge holder to the injector, we recommend using one-piece PEEK column fitting (Cat# HT6002) - it will adjust to any seal, depth or style and is reusable .Secure-Fit fittings, also available in both PEEK

and stainless steel, also are an excellent choice for consistent, leak-free seals. The guard cartridges are available in packs of 3 or 5.Guard cartridges are available in 10mm long and 2, 3, 4 and 4.6mm id. These cartridges can be supplied packed with all available materials in the Capital HPLC range .The unique design of end plugs ensure that there will not to be any leaks during the high pressure operation.

 

Ordering Information

 

 Part No.                     Description

 HT5171.1                  Universal Integral Cap-Guard

 To order a 10mm guard cartridge with a specific material, use the normal column part number (as

 described in the Capital HPLC order code guide on page 6) and replace the column length with 101.

 

 

 

 

 

 

 

 

HPLC Column Selection Table

Column Selection by USP Specifications

USP

Particle

Diameter

Bonded

Phase

Description

Capital

Recommendation

         

L1

3to 10

C18

Octadecyl silanechemically bondedto poroussilica

OptimalODS-H,Inertsil

     

or ceramicparticles.

ODS-3

L2

30to 50

C18

Octadecyl silanechemically bondedtosilicagel ofa

OptimalODS-H,40µm

     

controlledsurface porousitythathasbeen bondedto

 
     

asolidspherical cor e.

 

L3

1.5 to10

None

Poroussilicamicroparticles

OptimalSilica,Nucleosil

       

Silica, InertsilSi

L4

30to 50

Silica

Silica gelofcontrolledsurfaceporositybondedtoa

Optimalsi-40

     

solidsphericalcore.

 

L5

30to 50

Alumina

Aluminaof con trolledsurface porositybondedt oa

Call

     

solidsphericalcore.

 

L6

30to 50

Cation

Strongcation-exchangepacking-sulfonated

NucleosilSA,Spherisil

   

Exchange

fluorocarbonpolyme rcoatedonasolid sph ericalcore.

SAX,HypersphereSAX

L7

5to 10

C8

Octyl silanechemically bondedto totallyporous

OptimalC8 ,

     

microsilica particles.

HypersphereC8,InertpakC8

L8

10

NH2

An essentiallymonomolecular layerof

OptimalNH2 ,Nucleosil

     

amino propylsilanechemically bondedtotot ally

NH2,InertpakNH2

     

poroussilica gelsup port.

 

L9

10

Cation

Irregular,totallyporoussilicagel having achem ically

PartisilSCX,10 µm

   

Exchange

bonded,stronglyac id iccation-exchangecoating.

 

L10

5to 10

Nitrile

Nitrilegroupschemically bondedto poroussilica

OptimalCN,5µm,

     

microparticles.

HypersphereCN

L11

5to 10

Phenyl

Phenylgroups chemicallybondedtoporoussilica

OptimalPhenyl,

     

particles.

SpherisilPhenyl,

       

HyperspherePhenyl

L12

30to 50

Anion

Stronganion-exchangepacking-quaternary amine

HypersphereSAX,

   

Exchange

bondedonasolidsphericalcore.

SperisilSAX

L13

5to 10

Trimethylsilan

Trimethylsilan echemically bondedtoporous silica

SASHypersphere,5µm

   

e

microparticles.

 

L14

10

Anion

Silica gelhavinga chemicallybonded,stronglybasic

ExsilSAX,Hypersphere

   

Exchange

quaternaryam moni umanion-exchangecoat ing.

SAX,SperisilSAX

L15

3to 10

C6

Hexylsilane chemicallybondedto totallyporous

HexylHypersphere,

     

silicaparticles.

SpherisilC6

L16

5to 10

Dimethylsilan

Dimethylsilanechemicallybonded toporoussilica

ApexC2, 5µm

L17

7to 11

e

Cation

particles.

Strongcation-exchangeresinconsistingof

Nucleogel810H

   

Exchange

sulfonatedcross-linkedstyrene-divinylbenzene

 
     

copolymerinthe hydrogen form.

 

L18

5to 10

NH2/CN

Amino andcya nogroupschemically bondedto

Call

     

poroussilica particles.

 

L19

9

Cation

Strongcation-exchangeresinconsistingof

Nucleogel810Ca,Call

   

Exchange

sulfonatedcross-linkedstyrene-divinylbenzene

 
     

copolymerinthe calciumform.

 

L20

5to 10

Dihydroxypro

Dihydroxypropanegroups ch emicallybondedto

ShodexProteinKW-800,

   

pane

poroussilica particles.

InersilHilicProsphere

Hilic,Call

L21

5to 10

None

Arigid,sphericalstyrene-divinylbenzenecopolymer.

PRPHamiltonseries,Call

L22

10

Cation

Cation-exchangeresin madeof pououspolystyrene

PRP-X400Hamilton,

   

Exchange

gel withsulfonicacid groups, about10uminsize.

Call

L23

10

Anion

Anionexchangeresinmadeof porous

PRP-X500Hamilton,

   

Exchange

polymethacrylateor polyacryla tegelwithqu aternary

ShodexIECQA-825,

     

ammoniymgroups.

Call

L24

32to 63

None

Asemi-rigid hydrophilicgelconsistingofvinyl

ToyopearlHW40F1,

     

polymers withnumerous hydroxylgroupson thematrixsurface.

Call

L25

not

None

Packing having thecap acity tosepa ratecom pounds

ShodexOHpak SB-

 

specified

 

witha molecular rangefrom 100to50 00(as

802.5HQ,GPC 500

     

determine dbypolyethyleneoxide),applied to

Nucleogel, Call

     

neutral,anionic,and ca tionic wat er-soluble

 
     

polymers.Apolymethacrylate resinbase,cross-

 
     

linkedwith polyh ydroxylatedether(surface

containedsomeresidualcarboxyl functionalgroups)

 
     

wasfound su itable.

 

L26

5to 10

C4

Butylsilanechemicallybondedto totallyporous

OptimalC4 ,InertpakC4