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Category: p1
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Published on 11 March 2013
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Capital HPLC Narrow Bore Columns
Why use 4.6mm i.d. columns ?
Over the past twenty or so years, the most popular analytical HPLC column format has been the 4.6mm i.d. column. At the time of its introduction, this choice of a 4.6mm diameter column was dictated mainly by the limitations of the analytical instrumentation available then. For efficient chromatography, the dispersion caused by external instrumentation must be small relative to the volume of the eluting peaks. One means of ensuring this to be the case, is to increase the effective peak volumes by increasing the column bore. However, with the improvements in instrumentation, particularly in the area of miniaturisation, it is now possible to reduce the column bore and still maintain the efficiency of the column. This approach, which is made possible by the low dispersion characteristics of modern HPLC instrumentation offers the chromatographer a number of significant advantages over the use of conventional 4.6mm diameter columns.
Capital Narrow Bore Columns
Capital HPLC are able to supply high performance narrow bore columns of 3.2, 2.1mm and 1.0mm i.d., in a conventional column format, packed with any of the materials from our vast range. In addition to the standard 4.6mm i.d. format, UCC cartridge inserts are available in both 3.2mm and 2.1mm i.d. formats. Both the 3.2mm and 2.1mm inserts are fully compatible with the standard UCC cartridge holder, so it is not necessary to purchase specific narrow bore holders. At present the UCC range is the only cartridge system available which supports 2.1mm i.d. inserts.
Solvent Consumption
The use of narrow bore columns leads to a considerable reduction in solvent consumption. From figure 1, which shows the relative solvent consumption for 4.6, 3.2, 2.1 and 1.0mm i.d. columns or cartridges, it can be seen that even a moderate reduction in diameter from 4.6 to 3.2 can lead to a 50% saving in solvent costs. Not only does this save on solvents, but also on time spent preparing mobile phases and cuts waste disposal costs. As can be seen from the diagram below, the use of 1.0mm diameter columns results in a 95% saving in solvent costs. With such savings in the costs of solvent, it is even possible to consider the use of more exotic mobile phases containing expensive additives, such as chiral selectors.
Perhaps the most dramatic effect of reducing the cartridge diameter is the increased mass sensitivity. In situations where only a very small sample volume is available, it is often possible to obtain a five fold increase in sensitivity by changing from a traditional 4.6mm cartridge to a 2.1mm i.d.. A twenty fold increase in sensitivity can be achieved by switching from a 4.6mm to a 1.0mm i.d. column.
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Capital 3.2mm i.d. and 2.1mm i.d. Columns and Cartridges
Capital HPLC can supply 3.2mm and 2.1mm bore version of all the standard analytical columns in
our range and no extra cost. For ordering information,please consult the relevant section and
add D3 and D2 to the end of the standard column catalogue number in order to specify 3.2mm i.d.
and 2.1mm i.d. respectively.
Quality 1mm bore high efficiency columns are available packed with any of the materials and
phases in our range. Those listed below represent only a small selection of the more popular
columns. For columns not listed please contact our sales office.
Inertpak 1.0mm i.d. Narrow Bore Columns
5μm prices only
Phase 5cm 10cm 15cm 20cm 25cm |
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Silica 5MA105D1 5MA110D1 5MA115D1 5MA120D1 5MA125D1 |
C4 5MD105D1 5MD110D1 5MD115D1 5MD120D1 5MD125D1 |
C8 5MF105D1 5MF110D1 5MF115D1 5MF120D1 5MF125D1 |
ODS-2 5MG105D1 5MG110D1 5MG115D1 5MG120D1 5MG125D1 |
Phenyl(Ph) 5MK105D1 5MK110D1 5MK115D1 5MK120D1 5MK125D1 |
ODS(80Å) 5ML105D1 5ML110D1 5ML115D1 5ML120D1 5ML125D1 |
Price/£ see price list page 16-17 |
Optimal 1.0mm i.d. Narrow Bore Columns
5μm prices only, 3μm also available at 10% extra (please replace the intial 5 with 3 to specify)
Phase 5cm 10cm 15cm 20cm 25cm |
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ODS-H 5SG105D1 5SG110D1 5SG115D1 5SG120D1 5SG125D1 |
ODS-L 5SH105D1 5SH110D1 5SH115D1 5SH120D1 5SH125D1 |
Octyl 5SF105D1 5SF110D1 5SF115D1 5SF120D1 5SF125D1 |
Silica 5SA105D1 5SA110D1 5SA115D1 5SA120D1 5SA125D1 |
Price/£ see price list page 16-17 |
Hypersil 1.0mm i.d. Narrow Bore Columns
5μm prices only, 3μm also available at 10% extra (please replace the intial 5 with 3 to specify)
Phase 5cm 10cm 15cm 20cm 25cm |
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Si 5AA105D1 5AA110D1 5AA115D1 5AA120D1 5AA125D1 |
BUTYL(C4) 5AD105D1 5AD110D1 5AD115D1 5AD120D1 5AD125D1 |
MOS (C8) 5AF105D1 5AF110D1 5AF115D1 5AF120D1 5AF125D1 |
ODS (C18) 5AG105D1 5AG110D1 5AG115D1 5AG120D1 5AG125D1 |
Phenyl (Ph) 5AK105D1 5AK110D1 5AK115D1 5AK120D1 5AK125D1 |
CPS (CN) 5AL105D1 5AL110D1 5AL115D1 5AL120D1 5AL125D1 |
APS (NH2) 5AM105D1 5AM110D1 5AM115D1 5AM120D1 5AM125D1 |
BDS C18 5AZ105D1 5AZ110D1 5AZ115D1 5AZ120D1 5AZ125D1 |
BDS C8 5AY105D1 5AY110D1 5AY115D1 5AY120D1 5AY125D1 |
SAS (C1) 5AB105D1 5AB110D1 5AB115D1 5AB120D1 5AB125D1 |
Price/£ see price list page 16-17 |