Capital HPLC

Capital Optimal

The Next Step Forward in Silica Engineering

 

The Next Step Forward in Silica Engineering The new range of Optimal® HPLC columns from Capital HPLC respresents the next step forward in porous silica engineering beyond the so-called new generation high purity materials.
The current new generation materials are based on an alternative synthesis route which yields a high purity product with excellent inertness characteristics. However, Optimal® is manufactured using an entirely new process based on well established, tried and tested silica production techniques coupled with new refining technologies which ultimately leads to a superior material combining the advantages of both the new and traditional approaches to silica manufacturing.

 
 Specifications

The fact that high purity Optimal is manufactured using well established silica engineering techniques means that a wide range of specifications are possible. As a result, we can supply Optimal in pore sizes ranging from 60Å to 1000Å with the most popular being 120Å. Moreover, Optimal is also available in particle sizes ranging from 3μm up to preparative grade sizes.

Shape:
Phases Available:
Particle Size:
Mean Pore Diameter:
Carbon Loading:
Specific Surface Area:
Spherical
ODS-H, ODS-L, C8 and Silica
3μm, 5μm and larger
120Å (300, 500 and 1000Å also available)
ODS-H (18%), ODS-L (15%)
320m2g-1. (120Å material)

Optimal® ODS-H

Optimal® ODS-H is the most generally applicable of the Optimal® phases and can be used for both specialist
applications involving acid and basic compounds or mixtures of both. Optimal® ODS-H is also particularly well
suited to general purpose method development.

         

   

The absence of active silanols in Optimal is illustrated by this
standard test mixture which includes both pyridine and phenol.
The lack of peak tailing clearly demonstrates the absence of
metallic impurities.

Column:                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:        40:60 Acetonitrile:Water
Temperature:        40°C
Flow Rate:             1ml/min
Detection:             UV at 254nm
                             1. Pyridine, 2. Phenol

 

   

Neutral silanol groups can still occasionally cause problems
with certain classes of compound. Theobromine, theophylline
and caffeine are particularly prone to this effect. In Optimal this
effect is elimated by full end capping.

Column:                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:        25:75 Methanol:Water
Temperature:        30°C
Flow Rate:             1ml/min
Detection:             UV at 254nm
                             

                             1. Theobromine, 2. Theophylline
                             3. Caffeine, 4. Phenol

 

Many compounds such as polyphenols interact irreversibly with the active sites on silica based reversed
phase materials. In the case of Optimal ODS-H this problem does not occur, which makes it possible
to analyse problematic compounds such as in the example below.

 

Column:
                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:        25:75 Methanol:Water
Temperature:        30°C
Flow Rate:             1ml/min
Detection:             UV at 254nm

                             

                             

 

 

Silianol Sensitive Vitamins

Column:                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:       15:85 Acetonitrile:Water
                             10mM Sodium Phosphate (pH5)

Temperature:        40°C
Flow Rate:             1ml/min
Detection:             UV at 254nm
                                                       

                             1. Thiamine Hydrochloride
                             2. Riboflavine

 

 

 

Tricyclic Antidepressives

Column:                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:       80:20 Methanol:Water
                             20mM Potassium Phosphate (pH 7)

Temperature:        40°C
Flow Rate:             1ml/min
Detection:             UV at 254nm
                                                                                  

                             1. Protriptyline
                             2. Nortriptyline
                             3. Amitriptyline

 

 

 

 

 

 

 Optimal® ODS-L

Optimal® ODS-L has a slightly lower carbon loading (15%) than the ODS-H and is useful for the analysis of a wide range of hydrophilic compounds which are normally not retained by other C18 based packing material.

One particular advantage is that even pure water can be used as the eluent without the need for any organic modifier. Highly polar samples only soluble in water may be separated by this packing material, e.g. sugars and other saccharides.

Optimal ODS-L is also as useful for analysing hydrophobic samples which are currently chromatographed on competitor C8 materials. Like ODS-H, this packing material is also fully endcapped. OptimalODS-L is also based on the same high purity silica as the Optimal ODS-H material which ensures the high degree of inertness and lack of peak tailing.

 

Fructo-Oligosaccharides

Column:                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:       100% Water (HPLC Grade)
Temperature:       40°C
Flow Rate:             1ml/min
Detection:             Refractive Index

                                                                                                      

                             1. Sucrose
                             2. Kestose
                             3. Fructotriose
                             4. Fructotetraose

 

 

 

 

 

 

 

Optimal Ordering Information

Catalogue numbers refer to 5μm materials 4.6mm column i.d. only. For 2.1mm or 3.2mm
diameter columns please add D2 and D3 respectively to the standard 4.6mm i.d. columns.
Prices for 2.1mm and 3.2mm i.d. columns are as for 4.6mm i.d.

New Precision Format Columns
5mm prices only (3μm and 10μm particles are also available)

 

        Phase               2cm          5cm          10cm          15cm         20cm         25cm
                              (guard)
        Silica            5SA102      5SA105      5SA110       5SA115      5SA120     5SA125
         C8                5SF102      5SF105      5SF110       5SF115      5SF120      5SF125
       ODS-H            5SG102     5SG105      5SG110       5SG115     5SG120       5SG125
       ODS-L            5SH102     5SH105      5SH110       5SH115      5SH120      5SH125
      PRICE/£          See price list page 1

 


UCCCartridgeFormatColumns
5µm prices only (3µmand 10µmparticles are also available)

 

        Phase               2cm          5cm          10cm          15cm         20cm         25cm
                              (guard)
        Silica             5SA102      5SA305      5SA310       5SA315     5SA320     5SA325
         C8                5SF102      5SF305      5SF310       5SF315       5SF320     5SF325
       ODS-H            5SG102     5SG305      5SG310       5SG315      5SG320     5SG325
       ODS-L            5SH102      5SH305      5SH310       5SH315      5SH320     5SH325
      PRICE/£          See price list page 1

 

 

 Wide Pore Materials


All Capital HPLC Optimal columns are available in various pore sizes ranging from 60Å up to
1000Å. This makes it possible to analyse larger molecules together with all the advantages of
a high purity, highly inert material. For prices and ordering information for wide pore materials,
please consult our price list, page 1.