Capital HPLC

The Next Step Forward in Silica Engineering The new range of Optimal® HPLC columns from Capital HPLC respresents the next step forward in porous silica engineering beyond the so-called new generation high purity materials.
The current new generation materials are based on an alternative synthesis route which yields a high purity product with excellent inertness characteristics. However, Optimal® is manufactured using an entirely new process based on well established, tried and tested silica production techniques coupled with new refining technologies which ultimately leads to a superior material combining the advantages of both the new and traditional approaches to silica manufacturing.

The fact that high purity Optimal is manufactured using well established silica engineering techniques means that a wide range of specifications are possible. As a result, we can supply Optimal in pore sizes ranging from 60Å to 1000Å with the most popular being 120Å. Moreover, Optimal is also available in particle sizes ranging from 3μm up to preparative grade sizes.

The absence of active silanols in Optimal is illustrated by this
standard test mixture which includes both pyridine and phenol.
The lack of peak tailing clearly demonstrates the absence of
metallic impurities.

Column:                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:        40:60 Acetonitrile:Water
Temperature:        40°C
Flow Rate:             1ml/min
Detection:             UV at 254nm
                             1. Pyridine, 2. Phenol

Neutral silanol groups can still occasionally cause problems
with certain classes of compound. Theobromine, theophylline
and caffeine are particularly prone to this effect. In Optimal this
effect is elimated by full end capping.

Column:                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:        25:75 Methanol:Water
Temperature:        30°C
Flow Rate:             1ml/min
Detection:             UV at 254nm
                             

                             1. Theobromine, 2. Theophylline
                             3. Caffeine, 4. Phenol

Silianol Sensitive Vitamins

Column:                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:       15:85 Acetonitrile:Water
                             10mM Sodium Phosphate (pH5)

Temperature:        40°C
Flow Rate:             1ml/min
Detection:             UV at 254nm
                                                       

                             1. Thiamine Hydrochloride
                             2. Riboflavine

Tricyclic Antidepressives

Column:                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:       80:20 Methanol:Water
                             20mM Potassium Phosphate (pH 7)

Temperature:        40°C
Flow Rate:             1ml/min
Detection:             UV at 254nm
                                                                                  

                             1. Protriptyline
                             2. Nortriptyline
                             3. Amitriptyline

Optimal® ODS-L has a slightly lower carbon loading (15%) than the ODS-H and is useful for the analysis of a wide range of hydrophilic compounds which are normally not retained by other C18 based packing material.

One particular advantage is that even pure water can be used as the eluent without the need for any organic modifier. Highly polar samples only soluble in water may be separated by this packing material, e.g. sugars and other saccharides.

Optimal ODS-L is also as useful for analysing hydrophobic samples which are currently chromatographed on competitor C8 materials. Like ODS-H, this packing material is also fully endcapped. OptimalODS-L is also based on the same high purity silica as the Optimal ODS-H material which ensures the high degree of inertness and lack of peak tailing.

Fructo-Oligosaccharides

Column:                 Optimal® ODS-H 5μm
Dimensions:          15cm x 4.6mm
Mobile Phase:       100% Water (HPLC Grade)
Temperature:       40°C
Flow Rate:             1ml/min
Detection:             Refractive Index

                             1. Sucrose
                             2. Kestose
                             3. Fructotriose
                             4. Fructotetraose