Capital HPLC

Capital Optimal Bio

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Category: p1
Published on 11 March 2013
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 Capital Optimal® Bio

• Suitable for purification of small peptides and other compounds 
• 12nm pore size, narrow distribution range
• Totally spherical particles mare of ultra high purity silica gel
• Features extended acidic and alkalic

resistance and high durability The improved high density bonding and full endcapping makes it very suitable to separate or purify lowe molecular weight compounds, especially smaller peptides. Because of the significant improvement in durability and acidic/ alkalic resistance it can be used for extended periods of time in acidic mobile phase conditions and rinsed for recovery with an NaOH containing buffer.

The ODS phases are recommended for
general use: the first choice for unknown
compounds.

Superior Alkalic and Acidic resistance

  Alkalic Resistance

 Comparison of durability in Alkalic Environment
Size: 4.6mm I.D. x 150mm
Accelerated Alkalic Duration Test Condition
Mobile Phase: CH3CN/0.1N NaOH aq. (pH=13)=70/30;
Flow rate: 1.0ml/min; Temp.:40oC		 Chromatographic Test Condition
Mobile Phase: CH3OH/H2O=70/30; Flow rate:1.0ml/min;
Temp: 40oC; Detector: UV 254nm
1. Uracil 2. Methyl Benzoate, 3. Toluene 4. Naphthalene 

 

 Acidic Resistance

Size: 4.6mm I.D. x 150mm
Accelerated Acidic Duration Test Condition
Mobile Phase: CH3CN/H2O/TFA=70/30/1;
Flow rate: 1.0ml/min; Temp.: 70oC

 Chromatographic Test Condition

Mobile Phase: CH3OH/H2O=70/30; Flow rate:1.0ml/min;
Temp: 40oC; Detector: UV 254nm
1. Uracil 2. Methyl Benzoate, 3. Toluene 4. Naphthalene

 

 

 Conceptual Illustration of
 Theoretical Adsorption-Elution

Column Dimension: 4.6mm I.D x 250mm
Adsorption
Equilibration:          0.5% TFA aq
Solute:                  Human Insulin
Concentration:       10mg/ml
Feed:                    solute disolved in 0.5% TFA aq
Temp:                   30oC
Detector:              UV 290nm
Desorption/Elution
Equilibration:         0.5% TFA aq
Elution:                CH3CN/H2O/TFA = 30/70/0.1
Flow Rate:            0.5ml/min
Temperature:        30oC
Detector:              UV 300nm

 

 

Loading capacity of Insulin Pore
Diameter Correlation

Loading Capacity = F x Co x Tb / CV

F: Flow Rate
Co: Concentration
Tb: Breakthrough time
CV: Column Volume


 

 

 

Loading Capacity of Insulin
Breakthrough Curve Comparison

 

 

 

 

 

 

 

 

 

 

Alkalic Durability (pH=13)
Comparison of Si Dissolved
in Mobile Phase

Alkalic Durability Evaluation
Column Size:                4.6mm I.D.x 250mm
Mobile Phase:               Ethanol/0.1 N NaOH aq
(pH=13) =                    70/30
Flow Rate:                    2.0ml/min
Temperature:               ambient

Elution was collected every 50 CV and Si dissolved
was analysed by ICP.

 

 

 

 

 

 

 

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