Capital HPLC

USP

Particle

Diameter

Bonded

Phase

Des cription

Capital

Recom mendation

L1

3to10

C18

Octadecylsilanechemicallybondedtoporoussilica

OptimalODS-H,Inertsil

orceramicparticles.

ODS-3

L2

30to50

C18

Octadecylsilanechemicallybondedtosilicagelofa

OptimalODS-H,40µm

controlledsurface porousitythathasbeenbondedto

asolidsphericalcore.

L3

1. 5to10

None

P oroussilicamicroparticles

OptimalSilica,Nucleosil

Silica,InertsilSi

L4

30 to50

S ilica

S ilicagelofcontrolledsurfaceporositybondedtoa

Optimalsi-40

solidsphericalcore.

L5

30to50

Alumina

Aluminaofcontrolledsurfaceporositybondedtoa

Call

solidsphericalcore.

L6

30to50

Cation

Strongcation-exchangepacking-sulfonated

Nucle osilSA,Spherisil

Exchange

fluorocarbonpolymercoatedonasolidsphericalcore.

S AX,HypersphereSAX

L7

5to10

C8

Octylsilanechemicallybondedtototallyporous

OptimalC8,

microsilicaparticles.

HypersphereC8 ,InertpakC8

L8

10

NH2

Anessentiallymonomolecular layerof

OptimalNH2,Nucleosil

aminopropylsilanechemicallybond edtototally

NH2,InertpakNH2

poroussilicagelsupport.

L9

10

Cation

Irregular,totallyporoussilicagelhavingachemically

P artisilSCX,10µm

Exchange

bonded, stronglyacidiccation-exchangecoating.

L10

5to10

Nitrile

Nitrilegroupschem icallybondedtoporoussilica

OptimalCN,5µm,

microparticles.

HypersphereCN

L11

5to10

Phenyl

P henylgroupschemicallybondedtoporoussilica

OptimalPhenyl,

particles.

SpherisilPhenyl,

HyperspherePhenyl

L12

30to50

Anion

Stronganion-exchangepacking-quaternaryamine

HypersphereSAX,

Exchange

bonded onasolidsphericalcore.

SperisilSAX

L13

5to10

Trim ethylsilan

Trim ethylsilanechemicallybondedtoporoussilica

SASHypersphere,5µm

e

microparticles.

L14

10

Anion

Silicagelhavingachem icallybonded,stronglybasic

ExsilSAX,Hypersphere

Exchange

quaternaryammoniumanion-exchangecoating.

SAX,SperisilSAX

L15

3to10

C6

Hexylsilanechemicallybondedtototallyporous

HexylHypersphere,

silicaparticles.

S pherisilC6

L16

5to10

Dimethylsilan

Dimethylsilanechemicallybondedtoporoussilica

ApexC2,5µm

L17

7to11

e

Cat ion

p articles.

Strongcation-exchangeresinconsistingof

Nucle ogel810H

Exchange

sulfonated cross-linkedstyrene-divinylbenzene

copolymerinthehydrogenform.

L18

5to10

NH2/CN

Aminoandcyanogroupschemicallybondedto

Call

poroussilicaparticles.

L19

9

Cation

Strongcation-exchangeresinconsistingof

Nucle ogel810Ca,Call

Exchange

sulfonated cross-linkedstyrene-divinylbenzene

copolymerinthecalciumform.

L20

5to10

Dihydroxypro

Dihydroxypropanegroupschemicallybonded to

ShodexProteinKW-800,

pane

poroussilicaparticles.

InersilHilicProsphere

Hilic,Call

L21

5to10

None

A rigid,sphericalstyrene-divinylbenzenecopolymer.

PRPHamiltonseries,Call

L22

10

Cation

Cation-exchangeresinmadeofpououspolystyrene

PRP-X400Hamilton,

Exchange

gelwithsulfonicacidgroups,about 10uminsize.

Call

L23

10

Anion

Anionexchangeresinmadeofporous

PRP-X500Hamilton,

Exchange

polymethacrylateorpolyacrylategelwithquaternary

ShodexIECQA-825,

ammoniymgroups.

Call

L24

32 to63

None

A semi-rigidhydrophilicgelconsistingofvinyl

ToyopearlHW40F1,

polymerswithnumeroushydroxylgroupsonthematrixsurface.

Call

L25

not

None

Packinghavingthecapacitytoseparatecompounds

ShodexOHpakSB-

specified

withamolecularrangefrom100to5000(as

802.5HQ,GPC500

determinedbypolyethyleneoxide), appliedto

Nucle ogel,Call

neutral,anionic,andcationicwater-soluble

polymers.Apolymethacrylateresinbase,cross-

lin kedwithpolyhydroxylatedether(surface

containedsomeresidualcarboxylfunctionalgroups)

wasfoundsuitable.

L26

5to10

C4

Butylsilanechemicallybondedtototallyporous

OptimalC4,InertpakC4

USP

P ar ticle

Diameter

Bonded

Phase

Description

Capital

Recom mendation

L28

no t

C8/NH2

A multifunctionalsupport,whichconsistsofahigh

CapMixMod,call

specified

purity,100angstrom,spherical silicasubstratethat

hasbeenbondedwithanionic(amine)functionalityin

additiontoaconventionalreversedphase C8

functionality.

L29

5

Polybutadien

Gammaalumina,reversedphase,lowcarbon

Chromegabond,call

e

percentageby weight,alumina-basedpolybutadiene

spheric alparticles,withaporediameterof80

angstromunits.

L30

3to10

C2

Ethylsilanechemicallybondedtototallyporoussilica

ApexC2

particles.

L31

8. 5

Anion

Astronganion-exchangeresin-quaternaryamine

OMNIpacPAX-100-

Exchan ge

bondedonlatexparticles2000angstrom unitsand

Dionex,call

consistingofethylvinylbenzenecross-linkedwith55%

divinylbenzene.

L32

5to10

ChiralLigand

Achiral ligand-exchangepacking:L-prolinecopper

Nucleosil Chiral-1,call

complexcovalentlybondedExchangeto irregularly

shapedsilicaparticles.

L33

no t

None

Packinghavingthecapacitytoseparate proteinsby

ShodexKW-803,KW-

specified

m olecularsizeof4,000to400,000 daltons.Itis

804,call

spheric al,silica-based,overarangeand processed

toprovidepHstability.

L34

8to10

PSDVB-SCX

Strongcation-exchangeresinconsistingofsulfonated

Ham il tonHC-75 Pb2+

cro ss-l inkedstyrene-divinylbenzene copolymerinthe

leadform

L35

5to10

ZR-Diol

A zirconium-stabilizedsphericalsilica packingwitha

ZorbaxGF-250,call

hydrophilic(diol-type)molecular monolayerbonded

phasehavingaporesizeof150Å.

L36

5

Chiral

3,5-dinitrobenzoylderivativeofL-phenylglycine

Cap italChiral-1

covalentlybondedto5µmaminopropylsilica.

L37

5to10

GPC

Polymethacrylategelpackinghavingthe capacityto

ShodexOHpak SB-

separateproteinsbymolecularsizeovera rangeof

803HQ,Ultrahydrogel,

L38

5to20

Methacrylate-

2,000to40,000D.

Methacrylate-basedsize-exclusionpacking forwater-

call

ShodexOHpak SB-

SEC

solublesamples.

800HQ,Ultrahydrogel,

call

L39

5to10

Plymer

Hydrophilicpolyhydroxymethacrylategeloftotally

ShodexOHpak SB-

poroussphericalresin.

800HQ,Shode xRSpak

DM-614, Ultrahydrogel,

L40

5to20

Cellulosetris-3,5-dimethylphenylcarbamate coated

call

Cap italOD-H,

poroussilicaparticles,

CHIRALCELOD,call

L41

5

Glycoprotein

Imm obilizedα-acid glycoproteinonsphericalsilica

CHIRAL-AGP,call

particles,

L42

5

C8,C18

Octylsilaneandoctadecylsilanegroupschemically

OptimalODS-H,C8,

bondedtoporoussilicaparticles,

OptimalODS-L,C8,

Inertpak C8&C18

L43

5to10

Pentafl uorophenylgroupschemicallybondedtosilica

ChromegabondPFP,call

particles.,5to10µmindiameter.

L44

5to10

C8-SCX

A multifunctionalsupport,whichconsistsofahigh

ChromegabondRP-

purity,60Å, sphericalsilicasubstratethathasbeen

SCX,call

bondedwithacationicexchanger,sulfonicacid

functionalityinadditiontoaconventionalreversed

phaseC8functionality.

L45

5to10

β−Cyclodext

Betacyclodextrinbondedtoporoussilicaparticles

Cap italPak β-

rin

Cyclodextrin

L46

10

PSDVB-SAX

Polyst yrene/divinylbenzenesubstrate agglomerated

CarboPacPA1,

wit hquaternaryaminefunctionalizedlatexbeads.

CarboPacPA100,call

L47

8

SAX-NH2

Highcapacityanion-exchangemicroporoussubstrate,

CarboPacMA,call

fullyfunctionalizedwithatrimethylaminegroup

L48

15

PSDVB-SCX

Sulfo nated,cross-linkedpolystyrenewithanouter

IonPacAS7, call

layerofsubmicron,porous,anion-exchange

microbeads.

L49

3to10

Areversed-phasepackingmadebycoating athin

ZirchromPBD,Call

layerofpolybutadieneontosphericalporouszirconia

particles

USP

Particle Diamet er

Bonded

Phase

Description

Capital

Recommendation

L50

Multifunctionresinwithreversed-phaseretention

andstronganion-exchangefunctionalities.Theresin

OmniPacPAX-500,call

consistsofethylvinylbenzene,55%cross-linked

wi thdivinylbenzenecopolymer,3to15 µmin

diam et er,and asurfaceareaofnotlessthan350m2/g,substrateiscoatedwithquaternary

ammoniumfunctionalizedlatexparticlesconsisting

L51

5to10

Tris

ofstyrenecross-linkedwith divinylbenzene.Amylosetris-3,5-dimethylphenylcarbamate-coated,

ChiralpakAD,Call

carb om ate

porous,spherical,silicaparticles,

L52

5to10

SCX-Si

Astrongcationexchangeresinmadeofporous

TSKIC-Cation,TSKgel

sil icawithsulfopropylgroups,

SP-2 SW ,call

L53

5to10

W eakcation-exchangeresinconsisting of ethylvinylbenzene, 55%cross-linkedwith

IonPacCS14,call

divinylbenzene copolymer,3to15µmdiameter.

Substrateissurfacegrafted withcarboxylicacidand/orphosphoricacidfunctionalizedmonomers.

Capacitynotlessthan500 µmindiameter.

L54

13

SE C

Asizeexclusionmediummadeofcovalentbondingofdextrantohighlycross-linkedporousagarose

SuperdexpeptideHR

10/30,Call

beads.

L55

5

SCX-Polymer

Astrongcationexchangeresinmadeofporous

hypersphereSCX,

sil icacoatedwithpolybutadiene-maleicacid

Spheris ilSCX,IC-PakC

copolym er

M/D

L56

3to10

C3

Isopropylsilanechemicallybondedtototallyporous

Zorb axSBC-3,call

sil icaparticles.

L57

5

Ultron ES-

Achiral-recognitionprotein,ovomucoid,chemically

UltronES-OVM,Call

OV M

bondedtosilicaparticles,

L58

7to11

PSDVB-SCX

Strongcation-exchangeresinconsistingofsulfonatedcross-linkedstyrene-divinylbenzene