Capital HPLC

Column Selection by USP Specifications

Column Selection by USP Specifications

USP

Particle

Diameter

Bonded

Phase

Des cription

Capital

Recom mendation

         

L1

3to10

C18

Octadecylsilanechemicallybondedtoporoussilica

OptimalODS-H,Inertsil

     

orceramicparticles.

ODS-3

L2

30to50

C18

Octadecylsilanechemicallybondedtosilicagelofa

OptimalODS-H,40µm

     

controlledsurface porousitythathasbeenbondedto

 
     

asolidsphericalcore.

 

L3

1. 5to10

None

P oroussilicamicroparticles

OptimalSilica,Nucleosil

       

Silica,InertsilSi

L4

30 to50

S ilica

S ilicagelofcontrolledsurfaceporositybondedtoa

Optimalsi-40

     

solidsphericalcore.

 

L5

30to50

Alumina

Aluminaofcontrolledsurfaceporositybondedtoa

Call

     

solidsphericalcore.

 

L6

30to50

Cation

Strongcation-exchangepacking-sulfonated

Nucle osilSA,Spherisil

   

Exchange

fluorocarbonpolymercoatedonasolidsphericalcore.

S AX,HypersphereSAX

L7

5to10

C8

Octylsilanechemicallybondedtototallyporous

OptimalC8,

     

microsilicaparticles.

HypersphereC8 ,InertpakC8

L8

10

NH2

Anessentiallymonomolecular layerof

OptimalNH2,Nucleosil

     

aminopropylsilanechemicallybond edtototally

NH2,InertpakNH2

     

poroussilicagelsupport.

 

L9

10

Cation

Irregular,totallyporoussilicagelhavingachemically

P artisilSCX,10µm

   

Exchange

bonded, stronglyacidiccation-exchangecoating.

 

L10

5to10

Nitrile

Nitrilegroupschem icallybondedtoporoussilica

OptimalCN,5µm,

     

microparticles.

HypersphereCN

L11

5to10

Phenyl

P henylgroupschemicallybondedtoporoussilica

OptimalPhenyl,

     

particles.

SpherisilPhenyl,

       

HyperspherePhenyl

L12

30to50

Anion

Stronganion-exchangepacking-quaternaryamine

HypersphereSAX,

   

Exchange

bonded onasolidsphericalcore.

SperisilSAX

L13

5to10

Trim ethylsilan

Trim ethylsilanechemicallybondedtoporoussilica

SASHypersphere,5µm

   

e

microparticles.

 

L14

10

Anion

Silicagelhavingachem icallybonded,stronglybasic

ExsilSAX,Hypersphere

   

Exchange

quaternaryammoniumanion-exchangecoating.

SAX,SperisilSAX

L15

3to10

C6

Hexylsilanechemicallybondedtototallyporous

HexylHypersphere,

     

silicaparticles.

S pherisilC6

L16

5to10

Dimethylsilan

Dimethylsilanechemicallybondedtoporoussilica

ApexC2,5µm

L17

7to11

e

Cat ion

p articles.

Strongcation-exchangeresinconsistingof

Nucle ogel810H

   

Exchange

sulfonated cross-linkedstyrene-divinylbenzene

 
     

copolymerinthehydrogenform.

 

L18

5to10

NH2/CN

Aminoandcyanogroupschemicallybondedto

Call

     

poroussilicaparticles.

 

L19

9

Cation

Strongcation-exchangeresinconsistingof

Nucle ogel810Ca,Call

   

Exchange

sulfonated cross-linkedstyrene-divinylbenzene

 
     

copolymerinthecalciumform.

 

L20

5to10

Dihydroxypro

Dihydroxypropanegroupschemicallybonded to

ShodexProteinKW-800,

   

pane

poroussilicaparticles.

InersilHilicProsphere

Hilic,Call

L21

5to10

None

A rigid,sphericalstyrene-divinylbenzenecopolymer.

PRPHamiltonseries,Call

L22

10

Cation

Cation-exchangeresinmadeofpououspolystyrene

PRP-X400Hamilton,

   

Exchange

gelwithsulfonicacidgroups,about 10uminsize.

Call

L23

10

Anion

Anionexchangeresinmadeofporous

PRP-X500Hamilton,

   

Exchange

polymethacrylateorpolyacrylategelwithquaternary

ShodexIECQA-825,

     

ammoniymgroups.

Call

L24

32 to63

None

A semi-rigidhydrophilicgelconsistingofvinyl

ToyopearlHW40F1,

     

polymerswithnumeroushydroxylgroupsonthematrixsurface.

Call

L25

not

None

Packinghavingthecapacitytoseparatecompounds

ShodexOHpakSB-

 

specified

 

withamolecularrangefrom100to5000(as

802.5HQ,GPC500

     

determinedbypolyethyleneoxide), appliedto

Nucle ogel,Call

     

neutral,anionic,andcationicwater-soluble

 
     

polymers.Apolymethacrylateresinbase,cross-

 
     

lin kedwithpolyhydroxylatedether(surface

containedsomeresidualcarboxylfunctionalgroups)

 
     

wasfoundsuitable.

 

L26

5to10

C4

Butylsilanechemicallybondedtototallyporous

OptimalC4,InertpakC4

 

USP

P ar ticle

Diameter

Bonded

Phase

Description

Capital

Recom mendation

         

L28

no t

C8/NH2

A multifunctionalsupport,whichconsistsofahigh

CapMixMod,call

 

specified

 

purity,100angstrom,spherical silicasubstratethat

 
     

hasbeenbondedwithanionic(amine)functionalityin

 
     

additiontoaconventionalreversedphase C8

 
     

functionality.

 

L29

5

Polybutadien

Gammaalumina,reversedphase,lowcarbon

Chromegabond,call

   

e

percentageby weight,alumina-basedpolybutadiene

 
     

spheric alparticles,withaporediameterof80

 
     

angstromunits.

 

L30

3to10

C2

Ethylsilanechemicallybondedtototallyporoussilica

ApexC2

     

particles.

 

L31

8. 5

Anion

Astronganion-exchangeresin-quaternaryamine

OMNIpacPAX-100-

   

Exchan ge

bondedonlatexparticles2000angstrom unitsand

Dionex,call

     

consistingofethylvinylbenzenecross-linkedwith55%

 
     

divinylbenzene.

 

L32

5to10

ChiralLigand

Achiral ligand-exchangepacking:L-prolinecopper

Nucleosil Chiral-1,call

     

complexcovalentlybondedExchangeto irregularly

 
     

shapedsilicaparticles.

 

L33

no t

None

Packinghavingthecapacitytoseparate proteinsby

ShodexKW-803,KW-

 

specified

 

m olecularsizeof4,000to400,000 daltons.Itis

804,call

     

spheric al,silica-based,overarangeand processed

 
     

toprovidepHstability.

 

L34

8to10

PSDVB-SCX

Strongcation-exchangeresinconsistingofsulfonated

Ham il tonHC-75 Pb2+

     

cro ss-l inkedstyrene-divinylbenzene copolymerinthe

 
     

leadform

 

L35

5to10

ZR-Diol

A zirconium-stabilizedsphericalsilica packingwitha

ZorbaxGF-250,call

     

hydrophilic(diol-type)molecular monolayerbonded

 
     

phasehavingaporesizeof150Å.

 

L36

5

Chiral

3,5-dinitrobenzoylderivativeofL-phenylglycine

Cap italChiral-1

     

covalentlybondedto5µmaminopropylsilica.

 

L37

5to10

GPC

Polymethacrylategelpackinghavingthe capacityto

ShodexOHpak SB-

     

separateproteinsbymolecularsizeovera rangeof

803HQ,Ultrahydrogel,

L38

5to20

Methacrylate-

2,000to40,000D.

Methacrylate-basedsize-exclusionpacking forwater-

call

ShodexOHpak SB-

   

SEC

solublesamples.

800HQ,Ultrahydrogel,

       

call

L39

5to10

Plymer

Hydrophilicpolyhydroxymethacrylategeloftotally

ShodexOHpak SB-

     

poroussphericalresin.

800HQ,Shode xRSpak

       

DM-614, Ultrahydrogel,

L40

5to20

 

Cellulosetris-3,5-dimethylphenylcarbamate coated

call

Cap italOD-H,

     

poroussilicaparticles,

CHIRALCELOD,call

L41

5

Glycoprotein

Imm obilizedα-acid glycoproteinonsphericalsilica

CHIRAL-AGP,call

     

particles,

 

L42

5

C8,C18

Octylsilaneandoctadecylsilanegroupschemically

OptimalODS-H,C8,

     

bondedtoporoussilicaparticles,

OptimalODS-L,C8,

       

Inertpak C8&C18

L43

5to10

 

Pentafl uorophenylgroupschemicallybondedtosilica

ChromegabondPFP,call

     

particles.,5to10µmindiameter.

 

L44

5to10

C8-SCX

A multifunctionalsupport,whichconsistsofahigh

ChromegabondRP-

     

purity,60Å, sphericalsilicasubstratethathasbeen

SCX,call

     

bondedwithacationicexchanger,sulfonicacid

 
     

functionalityinadditiontoaconventionalreversed

 
     

phaseC8functionality.

 

L45

5to10

β−Cyclodext

Betacyclodextrinbondedtoporoussilicaparticles

Cap italPak β-

   

rin

 

Cyclodextrin

L46

10

PSDVB-SAX

Polyst yrene/divinylbenzenesubstrate agglomerated

CarboPacPA1,

     

wit hquaternaryaminefunctionalizedlatexbeads.

CarboPacPA100,call

L47

8

SAX-NH2

Highcapacityanion-exchangemicroporoussubstrate,

CarboPacMA,call

     

fullyfunctionalizedwithatrimethylaminegroup

 

L48

15

PSDVB-SCX

Sulfo nated,cross-linkedpolystyrenewithanouter

IonPacAS7, call

     

layerofsubmicron,porous,anion-exchange

 
     

microbeads.

 

L49

3to10

 

Areversed-phasepackingmadebycoating athin

ZirchromPBD,Call

     

layerofpolybutadieneontosphericalporouszirconia

 
     

particles

 

 

USP

Particle Diamet er

Bonded

Phase

Description

Capital

Recommendation

L50

   

Multifunctionresinwithreversed-phaseretention

andstronganion-exchangefunctionalities.Theresin

OmniPacPAX-500,call

     

consistsofethylvinylbenzene,55%cross-linked

 
     

wi thdivinylbenzenecopolymer,3to15 µmin

 
     

diam et er,and asurfaceareaofnotlessthan350m2/g,substrateiscoatedwithquaternary

 
     

ammoniumfunctionalizedlatexparticlesconsisting

 

L51

5to10

Tris

ofstyrenecross-linkedwith divinylbenzene.Amylosetris-3,5-dimethylphenylcarbamate-coated,

ChiralpakAD,Call

   

carb om ate

porous,spherical,silicaparticles,

 

L52

5to10

SCX-Si

Astrongcationexchangeresinmadeofporous

TSKIC-Cation,TSKgel

     

sil icawithsulfopropylgroups,

SP-2 SW ,call

L53

5to10

 

W eakcation-exchangeresinconsisting of ethylvinylbenzene, 55%cross-linkedwith

IonPacCS14,call

     

divinylbenzene copolymer,3to15µmdiameter.

 
     

Substrateissurfacegrafted withcarboxylicacidand/orphosphoricacidfunctionalizedmonomers.

 
     

Capacitynotlessthan500 µmindiameter.

 

L54

13

SE C

Asizeexclusionmediummadeofcovalentbondingofdextrantohighlycross-linkedporousagarose

SuperdexpeptideHR

10/30,Call

     

beads.

 

L55

5

SCX-Polymer

Astrongcationexchangeresinmadeofporous

hypersphereSCX,

     

sil icacoatedwithpolybutadiene-maleicacid

Spheris ilSCX,IC-PakC

     

copolym er

M/D

L56

3to10

C3

Isopropylsilanechemicallybondedtototallyporous

Zorb axSBC-3,call

     

sil icaparticles.

 

L57

5

Ultron ES-

Achiral-recognitionprotein,ovomucoid,chemically

UltronES-OVM,Call

   

OV M

bondedtosilicaparticles,

 

L58

7to11

PSDVB-SCX

Strongcation-exchangeresinconsistingofsulfonatedcross-linkedstyrene-divinylbenzene

ShodexKS-801,call

     

copolym erinthesodiumform,about7to11µm

 

L59

10

Processed

diam et er.

To separateproteinsbymolecularweightoverthe

NucleogelSEC,call

   

silica

rangeof10to 500kDa.Spherical10µm,silica-

 
     

based,andprocessedtoprovidehydrophilic

 

L60

3to10

 

characteristicsandpHstability.

Spherical,poroussilicagel,surfacehasbeen

Chromegabond,call

     

covalentlymodifiedwithpalmitamidopropylgroups

 
     

andendcapped.

 

L61

13

PSDVB-SAX

Hy droxide-selective,stronganion-exchangeresin

 
     

consistingofa highlycross-linkedcore , poresize

 
     

les sthan10Å,andconsistingofethylvinylbenzenecross-linkedwith55%divinylbenzenewithalatex

 
     

coatingcomposedof85nm diametermicrobeads

 
     

bondedwithalkanolquarternaryammoniumions(6

%)

 

L62

3to15

C30

C30silanebondedphaseonafullyporousspherical

ShodexKS-801,call

     

sil ica.

 

 

 

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