Capital HPLC

Waters Spherisorb

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Category: p1
Published on 11 March 2013
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Waters Spherisorb

Waters Spherisorb is a porous, spherical packing material manufactured by Phase Separations in Deeside, North Wales. Available in three, five and ten micron sizes, Spherisorb has a narrow size distribution and specific surface area and assures the chromatographer of a high efficiency and excellent peak shape characteristics. Waters Spherisorb in one of the more traditional phases and is widely used throughout the world.



 

SEPARATION OF PHENOLIC COMPOUNDS

Material: Spherisorb S5 ODS-1
Column: 250cm x 4.6mm
Mobile Phase: 70/30 Acetonitrile, Water
Flow Rate: 0.9 ml/min

Solutes

1. Phenol
2. P-Chlorophenol
3. O-Benzyl Chlorophenol
 

 

Specification
_________________________________________
 

3μm                   mean particle dia ±1 (dist 90%)
5μm                   mean particle dia ±2 (dist 90%)
10μm                 mean particle dia ±2 (dist 90%)

Average pore size 80Å
Pore size distribution 54 - 110Å
Surface area 220 m2g-1



Typical Chromatographic Performances
_________________________________________

3μm       - N = 100,000 p/m
5μm       - N = 55,000 p/m
10μm     - N = 35,000 p/m

 

 

 

 

 

 

 

 

CATECHOLAMINES

Column: Spherisorb S5C6
Eluent: 0.01M Phosphate, pH2.0
Detector: spectroMonitor III at 280nm

 

Peaks:


1. Noradrenalin
2. Adrenalin
3. Dopamine
4. Tyramine
5. Phenylethylamine

 

 
 

 

CARBOXYLIC ACIDS

1. Tartaric Acid
2. Lactic Acid
3. Malic Acid
4. Formic Acid
5. Acetic Acid
6. Citric Acid
7. Succinic Acid
8. Fumaric Acid

Sample
Volume: 10ul
Column Size: 250mm x 4.6mm
Packing: Spherisorb, Octyl
Bonded, Phase S5C8
Particle
Diam: 5 microns
Eluent: 0.2m H3 PO4
Aqueous
Pressure: 1200 psi
Temp: Ambient
Flow Rate: 0.8ml/min
Detector: UV 210nm
Sensitivity: 0.10 auf
 
 

 Ordering Information

Waters Spherisorb - Conventional Columns

5μm prices only
3μm columns 10% extra          - Replace first digit (i.e., 5) with 3 for cat. no.
10μm columns 5% less            - Replace first digit (i.e., 5) with 10 for cat. no.

 

   Phase                     2cm             5cm            10cm              15cm            20cm             25cm
                                (guard)
     Si                       5BA102        5BA105        5BA110        5BA115         5BA120         5BA125
     C1                      5BB102        5BB105        5BB110        5BB115          5BB120         5BB125
     C6                      5BE102        5BE105         5BE110        5BE115          5BE120         5BE125
     C8                      5BF102        5BF105         5BF110         5BF115          5BF120         5BF125
     ODS1                 5BG102        5BG105        5BG110         5BG115         5BG120         5BG125
     ODS2                 5BH102        5BH105        5BH110        5BH115         5BH120         5BH125
     PHENYL              5BK102        5BK105        5BK110         5BK115         5BK120         5BK125
     CN                      5BL102        5BL105        5BL110         5BL115          5BL120         5BL125
     NH2                   5BM102       5BM105        5BM110        5BM115        5BM120         5BM125
     SAX                    5BN102       5BN105         5BN110        5BN115        5BN120           5BN125
     SCX                    5BP102        5BP105         5BP110        5BP115         5BP120         5BP125

Waters Spherisorb - Universal Cartridge Columns (UCC)

5μm prices only
3μm columns 10% extra          - Replace first digit (i.e., 5) with 3 for cat. no.
10μm columns 5% less            - Replace first digit (i.e., 5) with 10 for cat. no.

   Phase                     2cm             5cm            10cm              15cm            20cm             25cm
                                (guard)
     Si                       5BA102        5BA305        5BA310        5BA315         5BA320         5BA325
     C1                      5BB102        5BB305        5BB310        5BB315          5BB320         5BB325
     C6                      5BE102        5BE305         5BE310        5BE315          5BE320         5BE325
     C8                      5BF102        5BF305         5BF310         5BF315          5BF320         5BF325
     ODS1                 5BG102        5BG305        5BG310         5BG315         5BG320         5BG325
     ODS2                 5BH102        5BH305        5BH310        5BH315         5BH320         5BH325
     PHENYL              5BK102        5BK305        5BK310         5BK315         5BK320         5BK325
     CN                      5BL102        5BL305        5BL310         5BL315          5BL320         5BL325
     NH2                   5BM102       5BM305        5BM310        5BM315        5BM320         5BM325
     SAX                    5BN302       5BN305         5BN310        5BN315        5BN320           5BN325
     SCX                    5BP302        5BP305         5BP310        5BP315         5BP320         5BP325

For a low cost alternative to Waters Spherisorb consider our Capital Spherisil (see page 12).

 

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